摘要
目的了解2013年江西省部分年龄<25岁的HIV感染者的亚型和原发耐药情况。方法随机抽取34份年龄<25岁HIV感染者的血浆样本,提取病毒RNA,采用RT-PCR和巢式PCR扩增HIV pol区和gag区基因片段,利用pol区基因序列测定结果分析HIV感染者原发耐药情况,同时利用gag区基因序列测定结果分析HIV感染者的亚型分布状况。结果 34位<25岁的HIV感染者的HIV亚型以CRF07_BC和CRF01_AE为主,分别占52.94%(18/34)和47.06%(16/34),且34份样本中未发现主要的监测性耐药突变。结论与2002年吸毒人群的HIV亚型流行状况相比,2013年江西省部分<25岁HIV感染者的亚型分布发生了显著变化,为进一步掌握江西省HIV-1亚型流行状况,应对近年来发现的HIV感染者进行分子流行病学调查。耐药株在<25岁的HIV感染者中的传播水平较低。
Objective To survey the subtype and transmitted drug resistance among part of HIV infections under 25 year - old in Jiangxi province in 2013. Methods 34 plasma samples were randomly collected from HIV infecters who were aged under 25 years old. Then, extracting RNA from plasma, the region of pol and gag genes were amplied by RT - PCR and nested PCR. The prevalence of transmitted drug resistance were analyzed from pol genes sequences, and meanwhile, subtype distribution was analyzed from gag genes sequences results. Results Among the subtypes of 34 HIV infections under 25 year - old, CRF07_BC （52.94% , 18/34） and CRF01 AE（47.06%, 16/34） were predominant, and none major surveillance drag resistance mutation was found. Conclusion Compared with the prevalence of HIV subtype among the drug addicfives in 2002, the subtype distribution of HIV infections under 25 years old in 2013 has changed. For further knowing the subtype distribution of HIV - l subtype in Jiangxi province, molecular epidemiology study should be conducted among HIV - 1 infected population diagnosed within the past years. The prevalence of HIV - I transmitted drug resistance among the newly infected with HIV who were aged under 25 years old was estimated as low.
出处
《中国卫生检验杂志》
CAS
2015年第9期1458-1460,共3页
Chinese Journal of Health Laboratory Technology
基金
江西省卫生计生委科技计划(20156017)
关键词
江西
原发耐药
亚型
Jiangxi
Transmitted drug resistance
Subtype
