摘要
目的建立同时测定冠心康颗粒中芍药苷、丹酚酸B和羟基红花黄色素A含量的方法。方法采用Welchrom C18色谱柱（250 mm×4.6 mm,5μm）,以乙腈（A）-0.1%磷酸（B）为流动相,梯度洗脱;流速：1.0 m L·min^-1;检测波长：230 nm。结果芍药苷在7.02-52.64μg·m L^-1内呈良好的线性关系（r=0.999 7）,平均回收率为99.3%,RSD为1.2%;丹酚酸B在23.19-173.90μg·m L^-1内呈良好的线性关系（r=0.999 7）,平均回收率为98.2%,RSD为1.1%;羟基红花黄色素A在4.47-33.50μg·m L^-1内呈良好的线性关系（r=0.999 3）,平均回收率为98.0%,RSD为1.0%。结论本法简便、灵敏、准确、重复性好,可用于冠心康颗粒的质量控制。
OBJECTIVE To establish an HPLC method for the simultaneous determination of chlorogenic acid, baicalin and baicalein in Yuxingcao Qinlan Mixtures. METHODS HPLC conditions included KromasiL^-100 C18（250 mm×4.6 mm, 5 μm） and the mobile phase was a mixture of methanol and 0.1% phosphoric acid for gradient elution. The flow rate was 1.0 m L·min^-1. The wavelength was 322 nm and column temperature was 35 ℃. RESULTS Three ingredients had good linear relationships： in the range of 0.022 4-1.12 μg（R2=0.999 7）, baicalin in the range of 0.045 8-2.29 μg（R2=0.999 5） and baicalein in the range of 0.031 8-1.59 μg（R2=0.999 9）. Their average recoveries were 99.45%（RSD=1.4%）, 99.37%（RSD=2.0%） and 97.90%（RSD=1.7%）, respectively. CONCLUSION This method was simple with high precision, good repeatability and stability. It could be used for the determination of the three ingredients and quality control of Yuxingcao Qinlan Mixtures.
出处
《中国现代应用药学》
CAS
CSCD
2015年第5期561-564,共4页
Chinese Journal of Modern Applied Pharmacy
基金
国家自然科学基金项目(81373668)
重庆市卫生局科技项目(2012-2-144)
