摘要
目的探讨miR-155在溃疡性结肠炎(UC)中的作用机制。方法构建miR-155高表达溃疡性结肠炎小鼠模型,并进一步采用Western blot方法检测外周血白细胞转录调节因子-1(EST-1)及Th17相关炎症因子蛋白表达。结果在UC模型组,miR-155基因转染后小鼠外周血白细胞中miR-155表达量较对照显著升高,且与UC结肠炎症程度呈正相关;UC模型组小鼠外周血白细胞中Est-1呈低表达,而Th17相关细胞因子(IL-17、IL-23及IL-6)呈相对高表达;miR-155转染后,UC小鼠这种变化趋势更加显著,Est-1表达更低,而IL-17、IL-23及IL-6表达更高。结论 miR-155通过抑制Ets-1表达,进而促进Th17相关炎症因子分泌参与溃疡性结肠炎的发病。
Objective To investigate the role of miR-155 in mice with ulcerative colitis（UC）. Methods The ulcerative colitis mice model with miR-155 high expression was established,E 26 transformation specific-1（Ets-1）and T helper 17 cells（Th17）associated cytokines expression was tested with Western blotting method. Results In UC mice group, the miR-155 expression in peripheral white blood cell（WBC） was significant higher after gene transfection and was positively associated with the severity of colitis. Est-1 in peripheral WBC was lowly expressed in UC mice,while Th17 associated cytokines IL-17,IL-23 and IL-6 were relatively highly expressed. After miR-155 gene transfection,the trend of lower expression of Est-1 and higher expression of IL-17、IL-23 and IL-6 was more obvious. Conclusion miR-155 is involved in the pathogenesis of UC by inhibiting Ets-1 expression, and further promoting Th17 associated inflammation cytokine excretion.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2015年第3期307-310,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省科技计划项目(2012B031800483)
