摘要
为了研究樟子松根部深色有隔内生真菌的耐盐性,笔者采用菌物学、生物化学等方法,研究了樟子松根部深色有隔内生(dark septate endophyte,DSE)真菌D575和D377在菌株生长和营养代谢方面对盐胁迫的响应。结果表明,在NaCl和Na2SO4胁迫条件下,菌株D575和D377的生长和营养代谢与盐浓度呈明显的相关性,不同盐浓度条件下菌株生长和营养代谢差异显著(P<0.05),在CNaCl≤0.2 mol/L和CNa2SO4≤0.2mol/L的低浓度盐胁迫条件下,D377和D575菌丝生长和对碳、磷相对利用率受抑制程度均较低,生长速率同对照相比相差不大,随着盐胁迫浓度的增高(CNaCl≥0.3mol/L和CNa2SO4≥0.3mol/L),菌株生长受到明显抑制,代谢缓慢。菌株间比较,DSE菌株D575对盐胁迫的耐受性好于菌株D377。
The paper aims to research the salt resistance of dark septate endophyte（DSE） fungus.Mycologyand biochemistry methods were adopted to research the salt tolerance of two dark septate endophyte fungusD377 and D575.The results showed that： under the stresses of NaCl and Na2SO4,the growth and nutritionalmetabolism were significantly correlated to salt concentrations.Under low salt concentration stress（CNaCl≤0.2 mol/L and CNa2SO4≤0.2 mol/L）,the inhibitions on strain growth and utilization rates of carbon and phosphorus were lower,and had no significant different compared to control.Under the high salt concentrationstress（CNaCl≥0.3 mol/L and CNa2SO4≥0.3 mol/L）,the inhibitions on strain growth and utilization rates of carbon and phosphorus were higher.DSE strain D575 had a better salt tolerance than D377.
出处
《中国农学通报》
2015年第13期36-42,共7页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目"深色有隔内生真菌与外生菌根菌互作对樟子松促生抗逆协同作用机制"(31200484)
"外生菌根菌与木霉互作对针叶苗木促生抗逆作用机制"(31170597)
国家"十二五"科技攻关项目专题"樟子松枯梢病微生物种群生态调控技术"(2012BAD19B0801)
黑龙江省森工总局项目"平贝土传病害生态控制技术"(SGZJY2012016)
"药用植物主要病虫害无公害防治技术"(SGZJY2010014)
关键词
深色有隔内生真菌
盐胁迫
菌丝生长
营养代谢
dark septate endophyte fungi
salt stress
mycelium growth
nutritional metabolism
