摘要
目的研究微波提取附子中多糖的最佳条件。方法利用微波提取法,在单因素试验的基础上,分别选取提取时间、提取温度、料液比3个因素的3个水平进行Box-Benhnken中心组合设计,以多糖提取率为因变量,利用响应曲面法对其提取工艺参数进行优化。结果最佳提取条件为:提取时间10 min,提取温度80℃,料液比1∶20。在此条件下,附子多糖提取率为16.10%。结论该工艺简便稳定、重现性好,可用于附子多糖的提取。
Objective To study the best condition of microwave-assisted extraction of polysaccharides from Radix Aconiti Lateralis Praeparata （Fuzi）. Methods Based on single factor experiment, a three-level, three-variable Box-Behnken design of response surface methodology adopting yield as response was applied in the optimization of the extracting parameters. Results The optimal conditions was determined as follows： extraction time was 10 min, extraction temperature was 80℃ and material/solvent ratio was 1：20. Under the optimized conditions, the extraction yield of Fuzi polysacchatides was 16.10%. Conclusion The extraction technology can be used for the extraction of polysaccharides from Fuzi.
出处
《中国医药导报》
CAS
2015年第16期48-51,共4页
China Medical Herald
基金
四川省卫生厅课题项目(130388)
关键词
附子
多糖
微波提取
响应曲面法
Radix Aconiti Lateralis Praeparata
Polysaccharides
Microwave-assisted extraction
Response surface method-ology
