摘要
目的探讨不同喂养方式婴儿肠道菌群分布特征。方法62例30～120d健康婴儿按喂养方式分为：母乳喂养、国产奶粉喂养、进口奶粉喂养及混合喂养4组。采集婴儿新鲜粪便标本。均分成2份，一份通过厌氧法分离培养并计数肠道双歧杆菌；另一份提取总DNA，通过肠杆菌基因问共有序列一PcR指纹图谱法寻找不同组间差异条带并进行克隆、测序及序列比对分析。结果母乳喂养组与混合喂养组肠道双歧杆菌数[（9．10±1．33）cug；（8．62±1．35）cfu/g]高于国产及进口奶粉喂养组[（7．62±1．22）cfu／g；（7．32±0．80）cfu/g，t=3．23，P〈0．05]；母乳喂养组与混和喂养组之间及2个奶粉喂养组之间差异无统计学意义。不同组间ERIC—PCR条带比对分析发现2条差异条带（A：1100bp；B：1000bp），其中A条带主要见于母乳、国产奶粉及混合喂养组，B条带主要见于进口奶粉组；测序及局部序列排比检索基本工具分析结果显示，A、B序列同源菌均为长双歧杆菌；序列编码产物A可能为糖代谢相关酶类；B可能为蛋白代谢相关酶类。结论母乳及混合喂养婴儿肠道中双歧杆菌数量多于奶粉喂养婴儿；国产奶粉喂养婴儿肠道中菌群分布更接近于母乳喂养婴儿。
Objective To investigate the characteristics of intestinal flora in infants with different feeding pat- terns. Methods Sixty - two cases of health infants （30 - 120 d） were divided into 4 groups according to their feeding patterns：breast feeding, imported powder milk feeding, domestic powder milk feeding and mixed feeding. Samples of their fresh feces in each group were collected and divided into sections equally：the bifidobacteria were isolated in anae- robic box and the number was counted for one section;for the other section, total DNA of intestinal flora was extracted and enterobacterial repetitive intergenic consensus （ ERIC ） fingerprints were amplified with the method of ERIC - PCR. After that, the specific bands observed in different groups were cloned and sequenced and alignmented. Results The colonies of bifidobacteria were more in breast feeding and mixed feeding groups[ （9.10 ±1.33） cfu/g; （8.62 ± 1.35） cfu/g]than those in domestic powder milk feeding and imported powder milk feeding groups[ （7.62 ± 1.22） cfu/g ; （ 7.32 ± 0.80 ） cfu/g, t = 3.23, P 〈 0.05 ] ; while there was no significant difference between breast feeding and mixed feeding groups, and between 2 powder milk feeding groups. Two specific bands were found from the ERIC finger- prints（ A：I 100 bp mainly in breast feeding, domestic powder milk feeding and mixed feeding groups;B：1000 bp main- ly in imported powder milk feeding group）. Sequencing and analysis of Basic Local Alignment Search Tool showed that homologous bacteria of A and B fragments were bifidobacterium longum. The encoding protein of A fragments might be related to the enzymes of carbohydrate metabolism, and B fragments were related to the enzymes of protein metabolism. Conclusions The colonies of bifidobacteria in intestinal tract are more in breast feeding and mixed feeding infants than those in formula feeding groups. The distribution of intestinal flora in domestic powder milk feeding infants is more similar to that of the breast feeding infants.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2015年第11期844-847,共4页
Chinese Journal of Applied Clinical Pediatrics
关键词
婴儿
肠道菌群
喂养方式
肠杆菌基因间共有序列.聚合酶链式反应
双歧杆菌
Infant
Intestinal flora
Feeding pattern
Enterobacterial repetitive intergenic consensus -polymerase chain reaction
Bifidobacteria
