摘要
目的:研究马栗籽提取物(EEHS)对衰老模型小鼠氧化损伤的改善作用。方法:采用sc给予D-半乳糖(120 mg/kg),每天1次,连续42 d以复制小鼠衰老模型。60只小鼠随机均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、维生素E(阳性对照,50 mg/kg)组与EEHS高、中、低剂量(300、150、75 mg/kg)组,后5组小鼠复制模型的同时ig给药,每天1次,连续42 d。测定小鼠血清、肝脏和脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、诱导型一氧化氮合酶(i NOS)活性与丙二醛(MDA)、一氧化氮(NO)含量。结果:与正常对照组比较,模型组小鼠血清、肝脏和脑组织中SOD、GSH-Px活性减弱,i NOS活性增强,MDA、NO含量增加,差异有统计学意义(P<0.01)。与模型组比较,EEHS高、中、低剂量组小鼠血清、肝脏和脑组织中SOD、GSH-Px活性增强,i NOS活性减弱,MDA、NO含量减少,差异有统计学意义(P<0.01或P<0.05)。结论:EEHS对衰老模型小鼠的氧化损伤有一定改善作用,其机制可能与增加机体抗氧化酶活性,提高清除自由基、抗氧化能力有关。
OBJECTIVE： To study the improvement effects of extract from hippocastanum seeds （EEHS） on the oxidative dam- age of aging model mice. METHODS： D-galactose （120 mg/kg） was sc given to mice by hypodermic injection once a day for con- secutive 42 d to establish aging models. 60 mice were randomly divided into normal control group （isometric normal saline）, mod- el group （isometric normal saline）, vitamin E group （positive control, 50 mg/kg）, and EEHS high, medium and low doses groups （300, 150, 75 mg/kg）. During the establishment of models, the drugs were given to the last five groups of mice, ig, once a day, for consecutive 42 d. The activities of the superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, inducible nitric oxide synthase （iNOS） and the contents of malondialdehyde （MDA） and nitric oxide （NO） in the serum, liver and brain tissues of the mice were determined. RESULTS ： Compared with normal control group, the activities of the SOD and GSH-Px in the serum, liv- ers and brain tissues of the mice in model group were decreased, the activity of the iNOS therein increased, and the contents of MDA and NO were increased, with significant difference （P〈0.01）. Compared with model group, the activities of the SOD and GSH-Px in the serum, livers and brain tissues of the mice in the EEHS high, medium and low dose groups were increased, the ac- tivity of the iNOS therein was decreased, and the contents of MDA and NO were decreased, with significant difference （P〈0.01 or P〈0.05）. CONCLUSIONS： EEHS has certain improvement effects on the oxidative damage of the aging model mice by a mech- anism that may be related to the enhancement of the activity of antioxidant enzyme, the elimination of free radical and the improve- ment in oxidation resistance.
出处
《中国药房》
CAS
北大核心
2015年第16期2175-2177,共3页
China Pharmacy
基金
甘肃省自然科学基金资助项目(No.145RJZA051)
关键词
马栗籽提取物
D-半乳糖
衰老
小鼠
氧化损伤
抗氧化作用
Extract from hippocastanum seeds
D-galactose
Aging
Mice
Oxidative damage
Antioxidant effects
