摘要
运用方差分析、主成分分析和聚类分析3种多元统计分析方法对团头鲂、长春鳊及其杂交子代(团头鲂♀×长春鳊♂)的形态学差异和性腺发育进行比较分析,结果表明:杂交子代的多数可数、可量性状同父母本相近,差异不显著;聚类分析显示杂交子代在形态上与母本团头鲂更相似,但遗传了父本长春鳊的全腹棱特征。对1龄和2龄杂交子代的性腺观察结果显示:1龄杂交子代性腺均呈细线状,肉眼很难分辨雌雄,组织切片观察精巢和卵巢大都处于Ⅱ期和Ⅲ期状态;2龄杂交子代的卵巢和精巢存在单侧发育和两侧不均衡发育的情况,大多处于Ⅳ期阶段。
In the present study,the morphologic characteristics and gonadal development of the F1hybrids（Megalobrama amblycephala（♀）× Parabramis pekinesis（♂））were investigated and compared with the parents.Through variance analysis,principal component analysis and cluster analysis,it was found that there were no significant morphological differences between F1 fish and their parents（P0.05）.Cluster analysis showed that the F1 hybrids were more similar with the female M.amblycephalain morphology;however,they also inherited the whole abdominal edge feature of paternal P.pekinesis.The gonadal development of F1 hybrids at one and two years old was observed by macroscopic and microscopic examination.At one year old,most testis and oraries were at stageⅡ and Ⅲ.At two years old,most testis and oraries were developed at stage Ⅳ,and there were unilateral and uneven development of both ovaries and testis in F1 hybrids.This study would be beneficial to the breeding of M.amblycephala.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2015年第4期89-96,共8页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(31472271)
国家大宗淡水鱼类产业技术体系建设项目(CARS4-464-05)
中央高校基本科研业务费专项(2013PY066和2014PY013)
海大集团团头鲂育种专项
关键词
团头鲂
长春鳊
杂交子代
形态差异
性腺发育
Megalobrama amblycephala
Parabramis pekinesis
hybrid
morphological character-istics
gonadal development
