摘要
目的探讨干扰素诱导跨膜蛋白3（IFITM3）在结肠癌组织中的表达及其临床意义。方法采用实时定量PCR和免疫组化法检测正常结肠组织、结肠腺瘤和结肠腺癌组织中IFITM3mRNA和蛋白的表达水平，分析其与结肠癌患者临床病理特征及预后的关系。结果IFITM3mRNA在正常结肠组织、结肠腺瘤、未转移结肠腺癌和转移结肠腺癌组织中的表达水平分别为4．49±0．69、7．32±0．76、12．19±1．34和18．37±0．61，差异有统计学意义（P〈0．001）。IFITM3蛋白在正常结肠组织、结肠腺瘤、未转移结肠腺癌和转移结肠腺癌组织中的阳性表达率分别为3．9％（2／51）、11．3％（14／124）、19．0％（19／100）和69．0％（78／113）。IFITM3蛋白表达与肿瘤周围组织浸润、肝转移、结肠旁淋巴结转移、结肠系膜淋巴结转移、系膜根部淋巴结转移、腹网膜转移和美国癌症联合委员会（MCC）分期有关（均P〈0．05）。IFITM3蛋白阳性表达结肠癌患者的5年生存率为40．2％，IFITM3蛋白阴性表达结肠癌患者的5年生存率为88．8％，差异有统计学意义（P〈0．001）。结论IFITM3表达与结肠癌转移程度及预后有关。
Objective To investigate the expression of IFITM3 in colorectal carcinoma and its clinical significance. Methods 213 patients with colon ademocareinoma and 214 patients with colon adenoma treated by surgery in our hospital from March 2008 to June 2010 were included in this study. The levels of IFITM3 in normal colon nueosa, adenoma, and adenocareinoma tissues were detected by real-time PCR and immunoehemistry, and its relationship with metastasis and prognosis in 213 colorectal cancer patients was analyzed. Results The IFITM3 mRNA level in metastatic tumor group was 18.37 ± 0.61, significantly higher than that in the normal 4.49±0.69 and non-metastases groups （7.32±0.76;F= 460.380, P〈 0. 001 ）. The positive rate of IFITM3 protein expression in metastatic tumor group （69.0%） was significantly higher than that in the normal （ 3.9% ） , non-metastasies groups （ 19.0% ） and adenoma groups （11.3%）. Our clinical analysis confirmed that the IFITM3 expression was associated with peritumoral invasion, hepatic metastases, metastases of para-eolonie lymph nodes, mesocolonic lymph nodes and mesenteric root lymph nodes, omental metastasis and AJCC classification （ P 〈 0.05 ）. Furthermore, the survival curve analysis showed that patients with lower IFITM3 level expression had a higher 5-year survival rate （88.8%） than that in the patients with higher expression （40.2%,P〈0.001）. Conclusions IFITM3 expression has a positive correlation with metastasis and prognosis in patients with colorectal carcinoma.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2015年第5期352-355,共4页
Chinese Journal of Oncology
基金
国家自然科学基金青年科学基金（81300113）
关键词
结肠肿瘤
干扰素诱导跨膜蛋白3
肿瘤转移
预后
Colonic neoplasms
Interferon induced transmembrane protein 3
Neoplasmmetastasis
Prognosis
