摘要
目的研究低压电脉冲刺激对荷黑素瘤小鼠的抑瘤效应。方法建立BALB/c小鼠皮下B16F10黑素瘤小鼠模型,随机分为对照组与10、20、30 Hz电刺激组,电刺激组接受30 min/d、连续20 d电刺激。每日测量肿瘤体积,HE染色观察肿瘤组织学变化,分别采用实时荧光定量聚合酶链式反应检测肿瘤组织caspase-3和基质金属蛋白酶9(MMP-9)的mRNA水平,免疫组织化学法检测肿瘤组织caspase-3和MMP-9的蛋白表达。结果与对照组相比,7 d时电刺激30 Hz组肿瘤体积减小,20 d时,各电刺激组肿瘤体积减小,随时间延长抑瘤率增加;电刺激组肿瘤细胞核固缩、坏死较多;各电刺激组caspase-3 mRNA及蛋白质表达增加;MMP-9 mRNA及蛋白表达降低,caspase-3、MMP-9表达强度变化随着频率增大而增强。结论低压电刺激可上调caspase-3表达,抑制MMP-9表达,抑制肿瘤生长。
Objective To investigate the antitumor effect of the low-voltage electric pulses （LVEPs） on melanoma-bearing mice. Methods Tumor models were established by subcutaneously inoculating BALB/c mice with B16F10 melanoma cells. These mice were randomly divided into control group and treated groups which were exposed to electric pulses of different frequency 10, 20, 30 Hz for consecutive 20 days with 30 minutes per day. In every day, tumor size was measured, and morphological changes of tumors were observed by HE staining. The expressions of caspase-3 and matrix metailoproteinase-9 （MMP-9） mRNAs were tested by real-time quantitative PCR, and the expressions of caspase-3 and MMP-9 proteins were detected by immunohistochemistry. Results Compared with the control group, seven days after LVEPs treatment, the tumor size in 30Hz treated group was reduced significantly; twenty days after treatment, the tumor size of all treated groups diminished significantly. The tumor growth inhibition rate increased with time. Compared with the control group, nuclear shrinkage and necrosis of more tumor cells were found in the treated groups; the expression of caspase-3 mRNA and protein was significantly enhanced in the treated groups; the expression of MMP-9 mRNA and protein was significantly depressed in the treated groups; and the changes of caspase-3 and MMP-9 expressions became greater with the frequency increasing. Conclusion LVEPs can induce the activation of caspase-3, suppress the expression of MMP-9, and inhibit tumor growth.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2015年第5期652-656,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
重庆市卫生局科研项目(2013-1-010)
关键词
低压电脉冲
肿瘤动物模型
抑瘤效应
免疫组织化学
low-voltage electric pulses
tumor animal model
antitumor effect
immunohistochemistry
