摘要
为了进一步改良火龙果品种,提高产量,以红皮红肉型火龙果紫红龙幼嫩茎段为试材,通过不同植物生长调节剂及浓度配比筛选出适宜火龙果愈伤组织诱导、不定芽再生及生根成苗的培养基,以建立稳定、快速、高效的火龙果再生体系。结果表明:高浓度的TDZ有利于茎段愈伤组织的形成,但不利于不定芽的再生,纵接比横接更利于愈伤组织和丛生芽的诱导,TDZ、2,4-D和NAA 3种生长调节剂通过正交试验筛选的愈伤组织和丛生芽诱导的最适培养基为:MS+2,4-D 1.0 mg·L-1+TDZ 0.4 mg·L-1+NAA 0.5 mg·L-1,愈伤组织诱导率为89.1%,丛生芽诱导率高达94.0%;生根诱导最适培养基为MS+CCC 0.5 mg·L-1+6-BA 3.0 mg·L-1+AC 200 mg·L-1,生根率达到97%,平均根数7.0条每株,平均根长达10.3 cm。
In order to further improve the pitaya varieties and yield, young stem segments of Zihonglong with red skin and red pulp were used as the test materials. Through selecting various plant growth regulators and concentration ratio, the culture medium, which was suitable for callus induction, adventitious bud regeneration and root formation, was screened to establish a stable, rapid and efficient plant regeneration system in pitaya （Hylocereus polyrhizus ＇Zihonglong＇ ）. The result indicated that high concentration of TDZ was beneficial to the callus induction, but not conducive to the adventitious bud regeneration. Vertical placement of the explants was better than horizontal placement for callus and adventitious shoot induction. The best medium for callus induction and adventitious shoot regeneration, through orthogonal designing with TDZ, 2,4-D and NAA, was MS medium supplemented with 1.0 mg·L^-1 2,4-D, 0.4 mg·L^-1 TDZ and 0.5 mg·L^-1 NAA, in which the callus induction rate was 89.1% and the adventitious shoot regeneration rate was 94% , The optimal medium for rooting was MS medium containing 0. 5 mg·L^-1 CCC, 3. 0 mg·L^-1 BA and 200 mg·L^-1 AC, which resulted in a rooting rate of 97% , the average root number of 7.0 and average root length of 10.3 cm.
出处
《核农学报》
CAS
CSCD
北大核心
2015年第5期885-891,共7页
Journal of Nuclear Agricultural Sciences
基金
国家公益性行业(农业)科研专项经费项目(201003073)
贵州省科技重大专项(20116010)
贵州省农科院创新基金(2011009)
关键词
火龙果
组织培养
愈伤组织
再生体系
pitaya, tissue culture, callus induction, plant regeneration
