摘要
目的:研究不同浓度的化学诱变剂甲基磺酸乙酯(Ethyl Methane Sulfonate,简称EMS)处理对加工番茄种子萌发的影响,筛选适宜的诱变浓度和诱变时间。方法:利用化学诱变剂EMS对加工番茄高代自交系"JW 9"的种子进行诱变处理,通过种子萌发试验,测定诱变后番茄种子的发芽势、发芽率、胚轴长、胚根长和侧根数,计算种子的发芽指数和活力指数。结果:EMS处理加工番茄"JW 9"的干种子时适宜浓度为4%,时间为12 h;EMS处理加工番茄"JW 9"的湿种子时适宜浓度为3%,时间为12 h。结论:EMS处理的种子(M0)受到的生理损伤随剂量增加而增加,湿种子更有利于EMS诱变剂的吸收,抑制作用更大,反应更剧烈。
Objective：This project aimed to study the effect of different concentrations of chemical mutagen EMS on processing tomato seed germination, chose appropriate mutagenesis concentration and mutagenesis time. Method：＂ JW 9＂, a high generation inbred lines variety, was mutated by chemical mutagenesis agent EMS, seed germinating and seeding growth were observed, the seeds germination potential, germination rate and hypoeotyl length, radicle length and number of lateral root were measured, the Seed germination index and vigor index were calculated. Result ： The appropriate concentration of EMS treatment processing tomato dry seed ＂JW9＂ was 4% EMS for 12 h. The appropriate concentration of EMS treatment processing tomato wet seed ＂JW 9＂ was 3% for 12 h. Conclusion ： The physical damage of seeds treated by EMS （ M0） increased with the increase of EMS concentration, the absorption of EMS mutagen was more effective for wet seeds, and the inhibition was larger , the reaction was more intense.
出处
《种子》
北大核心
2015年第5期28-33,共6页
Seed
基金
石河子大学"加工番茄新品种选育及诱变育种技术研究"(编号:gxjs 2012-yz 08)
新疆生产建设兵团科技局科技支疆计划项目"甲基磺酸乙酯(EMS)诱变加工番茄突变体及种质资源创新"(编号:2014 AB 004)
关键词
加工番茄
化学诱变
甲基磺酸乙酯
processing tomato
chemical mutagenesis
ethyl methane sulfonate
