摘要
目的分析香加皮的急性毒性与其中杠柳毒苷含量的相关性。方法 HPLC测定6批香加皮提取物中杠柳毒苷和4-甲氧基水杨醛的含量;小鼠分别灌胃给予6批香加皮提取物32 g膏/kg,4-甲氧基水杨醛200、250、300 mg·kg-1,观察14天内的毒性反应及死亡情况。结果小鼠灌胃给药后14天,香加皮125.7g生药/kg(含杠柳毒苷159.552mg·kg-1),106.6 g生药/kg(含杠柳毒苷112.032 mg·kg-1),111.7g生药/kg(含杠柳毒苷91.584 mg·kg-1),123.8 g生药/kg(含杠柳毒苷93.794 mg·kg-1),106.6 g生药/kg(含杠柳毒苷46.784 mg·kg-1),122.6 g生药/kg(含杠柳毒苷98.976 mg·kg-1)组死亡率分别为70%、20%、0、0、0、20%,用杠柳毒苷的量与死亡率做回归考察相关性,相关系数R2为0.9678;4-甲氧基水杨醛300 mg·kg-1组无死亡。结论柳毒苷含量与香加皮急性毒性实验结果有较好的相关性,杠柳毒苷含量的限定,可以较好的限制香加皮的毒性。
Objective To study the correlation of the content of periplocin with acute toxicity of Cortex Periplocae in different places. Methods The content determination of periplocin and 4-methoxy salicylic aldehyde in Cortex Periplocae was carried out by HPLC simultaneously. Mice were respectively orally administered with 32 g·kg^-1 of Cortex Periplocae extract, 4-methoxy salicylic aldehyde 200, 250, 300 mg·kg^-1, the toxic symptoms and the number of deaths were observed for fourteen days. Results The acute toxicity study showed that the mortality in the groups o^f Cortex Periplocae 125.7 g crude drug/kg（containing periplocin 159.552 mg·kg^-1）, 106.6 g crude drug/kg （Containing Periplocin 112.032 mg·kg^-1）, 111.7 g crude drug/kg （containing periplocin 91.584 mg·kg^ -1）, 123.8 g crude drug/kg （containing periplocin 93.794 mg·kg^-1）, 106.6 g crude drug/kg （containing periplocin 46.784 mg·kg^-1）, 122.6 g crude drug/kg （containing periplocin 98.976 mg·kg^-1） were 70%, 20%,0,0,0 and 20% respectively. Linear regression correlation analysis showed that the correlation coefficient was 0.9678. The mortality in the group of 4-methoxy salicylic aldehyde 300 mg·kg^-1 was 0. Conclusion There was good correlation between the content of periplocin and the results of the acute toxicity of Cortex Periplocae, the toxicity response can be reduced by limiting the content of periplocin.
出处
《中国药物警戒》
2015年第5期261-263,共3页
Chinese Journal of Pharmacovigilance
基金
"十一五"国家科技支撑计划重点项目(2006BAI14B01)
国家自然科学基金(81202850)
