摘要
目的:通过观察芪冬活血饮对脂多糖所致大鼠急性肺损伤(ALI)的干预作用,明确芪冬活血饮的保护作用并探讨其作用机制。方法:将50只SD大鼠随机分为空白组,模型组,高、中、低剂量中药治疗组5组。气道内滴注脂多糖建立急性肺损伤模型。中药治疗组以芪冬活血饮灌胃,空白组及模型组以0.9%氯化钠溶液灌胃。各组大鼠造模后24 h处死,收集标本。结果:1芪冬活血饮可以减轻肺损伤大鼠肺泡结构破坏,肺水肿及炎性细胞浸润。2ALI大鼠肺泡灌洗液肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-10水平增高,芪冬活血饮降低TNF-α、IL-1β水平,升高IL-10水平,高剂量组与低剂量组比较差异有统计学意义(P<0.01)。3ALI大鼠Toll样受体-4(TLR4)、核转录因子-κB(NF-κB)p65蛋白及m RNA表达增加,芪冬活血饮可减少TLR4、NF-κB p65蛋白及m RNA表达,并有一定的剂量相关性。结论:芪冬活血饮对LPS诱导的大鼠ALI有保护作用,其机制与抑制TLR4/NF-κB p65炎症信号通路,降低促炎细胞因子TNF-α、IL-1β水平,升高抗炎细胞因子IL-10水平有关。
Objective: To investigate the protective effect of qidonghuoxue decoction(QD) on acute lung injury(ALI) in rats induced by lipopolysaccharide(LPS), and to explore the mechanism. Methods: Fifty SD rats were divided into five groups randomly: control group, model group, high-dose QD group, medium-dose QD group, low-dose QD group. The ALI model was established by intratracheal instillation of LPS. QD was administrated via esophagus to rats in QD groups; NS was substitution in control and model groups. All rats were executed 24 h after modeling, bronchoalveolar lavage fluid(BALF) and lung tissue were collected. Results: 1HE staining showed that QD could reduce alveolar damage, pulmonary edema and inflammatory cells infiltration in rats with ALI. 2TNF-α, IL-1β, IL-10 levels were increased in rats with ALI, QD decreased the levels of TNF-α and IL-1β, increased the level of IL-10, there was significant difference between the high-dose and lowdose group(P〈0.01). 3The protein and m RNA expression of TLR4/NF-κB p65 both increased in rats with ALI, while QD reduced the expression,and the effect was kind of dose-related. Conclusion: QD has a protective effect on ALI induced by LPS in rats, its mechanism is related to inhibit TLR4/NF-k B p65 signaling pathway, decrease IL-1β/TNF-α levels and increase IL-10 level.
出处
《温州医学院学报》
CAS
2015年第5期332-337,共6页
Journal of Wenzhou Medical College
基金
国家自然科学基金资助项目(81273678)
浙江省自然科学基金资助项目(LQ12H29003)