摘要
目的:对《中国药典》中丹参药材含量测定方法进行优化。方法:以丹参酮ⅡA,丹酚酸B为指标,对《中国药典》丹参药材含量测定中供试品制备方法进行优化,采用正交试验法进行丹参提取工艺优选,用高效液相色谱法进行含量测定,丹参酮ⅡA以甲醇-水(85∶15)为流动相,检测波长270 nm;丹酚酸B以甲醇-乙腈-甲酸-水(30∶10∶1∶59)为流动相,检测波长286nm。结果:确定丹参酮ⅡA最佳提取工艺方案为:采用90%甲醇提取在75℃下回流2次,每次0.5 h;丹酚酸B最佳提取工艺方案为:使用70%甲醇提取在75℃下回流2次,每次1.5 h。结论:改进的提取工艺与测定方法更全面真实准确地反映丹参药材中丹参酮ⅡA和丹酚酸B的含量。
Objective： To optimize the method in the Chinese Pharmacopoeia for determining Salviae Miltiorrhizae Radix et Rhizoma. Method： Tanshinone ⅡA and salvianolic acid B were selected as the index in optimization of the sample preparation method of Salviae Miltiorrhizae Radix et Rhizoma in Chinese Pharmacopoeia. Orthogonal test was used to optimize the extraction process of Salviae Miltiorrhizae Radix et Rhizoma, and concentration of contents were detected by high performance liquid chromatography method. A detection of using methanol-water（85： 15） at wavelength of 270 nm was employed for tanshinone ⅡA, and a detection of using methanol- acetonitrile-formic acid-water （30： 10： 1： 59） at wavelength of 286 nm was employed for salvianolic acid B. Result： The optimized extraction process of tanshinoneⅡA and salvianolic acid B was： extracted by 90% methanol and reflux twice（0.5 h each time） at 75 ℃, extracted by 70% methanol and reflux twice（1.5 h each time） at 75 ℃, respectively. Conclusion： Optimized extraction and determination methods could be used to reflect the content of tanshinoneⅡA and salvianolic acid B in Salviae Miltiorrhizae Radix et Rhizoma more accurately and efficiently.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第11期2128-2131,共4页
China Journal of Chinese Materia Medica
基金
安徽道地中药材品质提升协同创新中心项目
中医药行业科研专项安徽种子种苗基地建设项目(201207002)
关键词
丹参
提取工艺
正交试验
含量测定
Salviae Miltiorrhizae Radix et Rhizoma
extraction process
orthogonal experimental design
content determination
