摘要
为了探讨苦参总碱、美洲大蠊提取物及两者联合用药对孕激素受体(PR)阴性的子宫内膜癌细胞株JEC的作用。笔者通过MTT法、流式细胞仪、Western blot检测苦参总碱、美洲大蠊提取物和两者联合用药后对JEC细胞的生长抑制、细胞周期及P53和c-erb B-2基因蛋白的表达影响。结果发现,1MTT法:苦参总碱和美洲大蠊提取物对JEC的生长均有抑制作用,且联合给药后抑制作用显著提高。2流式细胞仪分析:苦参总碱组细胞在G0/G1期的比例上升,美洲大蠊提取物组细胞在G2/M期比例上升,联合给药组的G0/G1期比例较其他2组显著上升,细胞生长受到抑制(P<0.05)。3Western blot法:苦参总碱组、美洲大蠊提取物组和联合给药组作用JEC细胞后,P53蛋白的表达水平上调,c-erb B-2蛋白表达水平下降(P<0.05)。以上结果说明苦参总碱、美洲大蠊提取物及联合给药对JEC细胞的生长均有抑制作用,且能通过上调P53和下调c-erb B-2蛋白的表达诱导细胞周期阻滞,抑制细胞增殖。
To study the effect of total matrines and extracts from Periplaneta americana on negative endometrial cancer cell JEC of progesterone receptors. After detecting the effect of total matrine, extracts from P. americana and their combination on JEC cells＇ growth inhibition, cell cycle, P53 and c-erbB-2 gene protein expressions through MTT, flow cytometry instrument and Western blot method, the author found that, ①MTT： total matrines and extracts from P. americana could inhibit the growth of JEC cell, with significant increase in the inhibitory effect in the combination group. ②Flow cytometry instrument： the cell cycle at G0/G1 increased after the treatment with total matrines, the cell cycle at G2/M increased after the treatment with extracts from periplaneta americana, and the ratio of G0/G1 cell cycle in the combination group was significantly higher than the other groups, with inhibition in cell growth and statistical difference in inter-group comparison （P〈0.05）. ③Western blot： the expression level of P53 increased and c-erbB-2 decreased after the treatment with total matrines, extracts from P. americana and their combination on JEC cell, with statistical difference in inter-group comparison （P〈0.05）. The above results suggested that total matrines, extracts from P. americana and their combination could induce cell cycle arrest and inhibit the growth of JEC cell by up-regulating P53 and down-regulating the c-erbB-2 level.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第11期2210-2213,共4页
China Journal of Chinese Materia Medica
