摘要
建立了超高效液相色谱-串联质谱联用技术同时测定配方奶粉中泛酸、生物素和氰钴胺素的方法。样品经水溶超声提取、三氯甲烷除蛋白后,进行超高效液相色谱-串联质谱分析,采用HSS T3液相色谱柱分离,以10mmol/L乙酸铵溶液（含0.1%甲酸）和乙腈作为流动相进行梯度洗脱,采用电喷雾-正离子电离源多反应监测模式进行定性和定量分析。结果表明：泛酸（VB5）的方法定量限为5μg/100g,生物素（VB7）为2μg/100g,氰钴胺素（VB12）为0.2μg/100g;回收率为79.4%~87.8%,相对标准偏差为3.3%~5.3%。泛酸的线性范围0.5~1000ng/m L,生物素为0.2~10ng/m L,氰钴胺素为0.02~10ng/m L。该方法分析操作简单、速度快、灵敏度高、重复性好,适用于配方奶粉中泛酸、生物素和氰钴胺素的同时测定。
Ultra performance liquid chromatography-tandem quadrupole mass spectrometry （UPLC-TQMS） method had been developed for the determination for pantothenic acid （VBs）, biotin（VBT）,cyanocobalamine（VB12） in formula milk powder. The samples were ultrasonic extracted by water,and precipitated protein by chloroform for analyzing by UPLC-MSMS. The analytes were separated by HSS T3 column. The mobile phase consisted of 10mmol/L ammonium acetate with 0.1% formic acid and acetonitrile. The samples were identified and quantified by multiple reaction monitoring （MRM） via positive electrospray ionization （ESI＋）. The results showed that the limit of quantification of VB5 were 5μg/100g, VB7 were 2μg/100g,VB12 were 0.2μg/100g. The recoveries at three spiked levels and the relative standard deviations were 79.4%-87.8% and 3.3%-5.3%,respectively. This method was simple,time saving,sensitive and accurate in the determination of VB5,VB7,VB12 in formula milk powder.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第12期61-64,共4页
Science and Technology of Food Industry
基金
北京市科学技术研究院青年骨干计划(201315)
北京市科技计划(Z141100002614013)
关键词
泛酸
生物素
氰钴胺素
超高效液相色谱-串联质谱法
奶粉
pantothenic acid
biotin
cyanocobalamine
ultra performance liquid chromatography-tandem quadrupole mass spectrometry
milk powder
