摘要
采用纳米沉淀法制备琼脂糖磁性纳米粒,并对其进行活化、偶联连接臂、螯合Ni2+离子,制备固定化金属离子磁性纳米粒(NS-ECH-IDA-Ni2+)。以NS-ECH-IDA-Ni2+为吸附剂,通过静态吸附试验研究NS-ECH-IDANi2+对牛血清蛋白(BSA)的吸附性能,并进行了吸附动力学和吸附等温线研究。结果表明:所得纳米粒球形圆整,平均粒径为850 nm,四氧化三铁含量为42.85%,环氧基修饰密度为72μmol/g,其Ni2+含量为58μmol/g。在试验条件下,吸附过程可用准二级动力学方程描述(R2=0.997 9~0.999 8)。p H是影响吸附作用的重要因素,当在p H约为9.0时,BSA的吸附量达到最大值。吸附等温曲线符合Langmuir等温吸附模型(R2=0.965 8)。
The agarose coated magnetic nanoparticles were prepared with nanoprecipitation method,then they were used to prepare immobilized metal affinity magnetic nanoparticles by being activated,coupled linkers and chelated nickel ions.The adsorption capacity of the nanoparticles for bovine serum albumin（BSA） was investigated through static adsorption test.In addition,the adsorption mechanism was determined using adsorption kinetics and adsorption isotherm.The results showed that the generated nanoparticles were round spheres which had an average diameter of 850 nm,an iron oxide content of 42.85%,an epoxy group density of 72 μmol/g and a nickel ion content of 58 μmol/g.Under experimental conditions,the BSA adsorption kinetics by nanoparticles fit the pseudosecond-order model.The pH has an important effect on BSA adsorption,and the adsorption capacity is the maximum at pH 9.0.The isothermal adsorption curve of nanoparticles fits the Langmuir model well（R^2=0.9658）.
出处
《食品工业》
CAS
北大核心
2015年第6期49-53,共5页
The Food Industry
基金
广西自然科学基金资助项目(2013GXNSFAA019040)
广西高等学校优秀人才资助项目(2012)41号
广西植物功能物质研究与利用重点试验室开放基金课题(FPRU2013-8)
广西生物炼制重点试验室开放课题(GXBF11-06)
关键词
磁性纳米粒
金属离子亲和
牛血清蛋白
吸附
magnetic nanoparticlcs
metal affinity
bovine serum albumin
adsorption
