摘要
目的:观察复方中药组分(黄连生物碱40%、三七总皂苷35%、麦冬多糖25%)对糖尿病模型大鼠血浆内皮素-1(ET-1)、血管紧张素Ⅰ(AngⅠ)、血管紧张素Ⅱ(AngⅡ)的影响。方法:用链脲菌素制备糖尿病大鼠模型,随机分为5组(模型对照组、二甲双胍组及高、中、低剂量中药组),每组各10只;另取10只作为空白对照组。其中模型对照组和空白对照组用0.9%Na Cl溶液10 ml·kg-1·d-1,二甲双胍组用二甲双胍0.05 mg·kg-1·d-1,高、中、低剂量中药组分别用中药组分0.45、0.15、0.05 g·kg-1·d-1,连续灌胃干预4周后断头采血;用酶联免疫吸附法检测血浆中ET-1、AngⅠ、AngⅡ的含量。结果:模型对照组ET-1、AngⅠ、AngⅡ含量明显高于空白对照组,差异均有统计学意义(P<0.05,P<0.01)。中、高剂量中药组和二甲双胍组ET-1、AngⅠ含量低于模型对照组,差异有统计学意义(P<0.05);高剂量中药组与二甲双胍组AngⅡ含量低于模型对照组,差异有统计学意义(P<0.05)。高剂量中药组AngⅡ含量低于二甲双胍组,差异有统计学意义(P<0.05)。结论:复方中药组分可能通过降低ET-1、AngⅠ和AngⅡ的水平减轻血管内皮损伤,起到改善糖尿病血管并发症的作用。
Objective： To observe the impact of multi-component of the compound traditional Chinese medicine （40% Alkaloids from Coptis Root ,35% Panax Notoginseng Saponins, 25% Polysaccharide from Ophiopogon japonicus） on ET-1, Ang Ⅰ and Ang Ⅱ in blood of diabetic nephropathy rat. Methods： The diabetic nephropathy rats model was established with streptozotocin, then divided randomly into model control group, metformin group, high-dose Chinese medicine group, mediumdose Chinese medicine group and low-dose Chinese medicine group, 10 rats in each group, another 10 rats were divided into blank control group. Model control group and blank control group used 0.9% NaCl solution 10 ml · kg^-1· d^-1 , mefformin group used metformin 0.05mg · kg^- 1 · d ^- 1, high- dose, mediumdose and low- dose Chinese medicine groups used multi- component of the compound traditional Chinese medicine 0.45,0.15,0.05 g · kg^-1· d^-1 respectively. Blood was collected after irrigating mice stomach successively for 4 months to detect content of ET- 1, Ang Ⅰ and Ang Ⅱ in blood by MonoLISA mothed. Results： Content of ET- 1, Ang Ⅰ and Ang Ⅱ in model control group was obviously higher than that in the blank control group, and there is a significant statistical difference between the two groups（ P 〈0.05 ,P 〈 0.01 ）. Content of ET-1, Ang Ⅰ in medium-dose, high-dose Chinese medicine groups and metformin group was lower than model control group, and there is a significantly statistical difference between the four groups （ P 〈 0.05 ）. Content of Ang H in high-dose Chinese medicine group and metformin group was lower than that in the model control group, and there is a significantly statistical difference between the three groups （ P 〈 0.05 ）. Content of Ang H in high-dose Chinese medicine group was lower than metformin group, and there is a significantly statistical difference between the two groups （ P 〈 0.05 ）. Conclusion ： Multi- component of the compound traditional Chinese medicine may have the effects of improving diabetic vascular complications though decreasing the content of ET- 1,Ang Ⅰ and Ang Ⅱ to lighten vascular endothelial injury.
出处
《上海中医药大学学报》
CAS
2015年第3期66-69,共4页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(81173617)
55批中国博士后科学基金面上资助项目(2014M551000)
关键词
中药组分
糖尿病
内皮素-1
血管紧张素Ⅰ
血管紧张素Ⅱ
大鼠
multi-component of the compound traditional Chinese medicine
Diabetes Mellitus
ET-1
Ang Ⅰ
AngⅡ
rat
