摘要
目的探讨血管内皮生长因子（VEGF）、血管形成蛋白1（Ang-1）和Ang-2对人源性心脏微血管内皮细胞（HCMEC）的神经调节蛋白1（NRG-1）表达和NRG-1β分泌的影响。方法培养HCMEC至4～5代,在正常培养条件下,将其分为正常组、VEGF处理组、Ang-1处理组和Ang-2处理组,与HCMEC共孵育24h,收集细胞和培养液,用蛋白印迹法和细胞酶联免疫吸附法分别检测NRG-1蛋白表达和NRG-1β分泌。蛋白印迹法检测酪氨酸激酶受体（ErbB2、ErbB3和ErbB4）蛋白表达。结果ErbB2、ErbB3和ErbB4均表达于HCMEC。与正常组比较,VEGF处理组和Ang-1处理组NRG-1[（1.44±0.05）、（1.18±0.04）vs（1.05±0.06）]表达和NRG-1β[（534.07±6.89）ng/L、（505.19±22.82）ng/L vs（380.71±14.96）ng/L]分泌显著增加,差异有统计学意义（P〈0.05）,而Ang-2处理组NRG-1表达和NRG-1β分泌显著降低（P〈0.05）。结论VEGF和Ang-1显著增加HCMEC的NRG-1表达和NRG-1β分泌,Ang-2显著减少HCMEC的NRG-1表达和NRG-1β分泌。
Objective To study the effect of VEGF,Ang-1and Ang-2on neuregulin-1（NRG-1）expression and NRG-1βsecretion in human cardiac microvascular endothelial cells（HCMEC）.Methods HCMEC were cultured to their 4-5generation and divided into normal control group,VEGF treatment group,Ang-1treatment group and Ang-2treatment group.NRG-1expression and NRG-1βsecretion in HCMEC were detected by Western blot and ELISA,respectively.Expressions of ErbB2,ErbB3 and ErbB4protein were detected by Western blot.Results ErbB2,ErbB3 and ErbB4were expressed in HCMEC.The NRG-1expression level and the NRG-1βsecretion level were significantly higher in VEGF treatment group and Ang-1treatment group than in normal control group（1.44±0.05 vs 1.05±0.06,1.18±0.04 vs 1.05±0.06;534.07±6.89ng/Lvs 380.71±14.96ng/L,505.19±22.82ng/Lvs 380.71±14.96ng/L,P〈0.05）and significantly lower in Ang-2treatment group than in normal control group（P〈0.05）.Conclusion VEGF and Ang-1upregulate the NRG-1expression and the NRG-1βsecretion while Ang-2downregulates the NRG-1expression and the NRG-1βsecretion in HCMEC.
出处
《中华老年心脑血管病杂志》
CAS
2015年第6期630-633,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
国家自然科学基金(81160021
81160042
81160046)
广西自然科学基金重点课题(2014GXNSFDA118024)
