摘要
通过考察实验的稳定性、精确度及回收率等,研究不同氨基酸对牛磺酸检测的干扰,成功建立了离子色谱法分离牛磺酸梯度洗脱程序,探索出一种更高效、更快速、更灵敏的离子色谱检测方法。色谱柱为氨基酸分析柱(Amino Pac PA-10,2 mm×250 mm)、氨基酸保护柱(Amino Pac PA-10 2 mm×50 mm);流动相组成为流动相A(纯水)、流动相B(250 mmol/L Na OH溶液),流动相C(1.0 mmol/L乙酸钠(Na OAc)),进行梯度洗脱;流速为0.24m L/min;柱温为30℃;采用积分脉冲安培检测器检测;进样量为25μL。该法优化了牛磺酸的检测时间,提高了牛磺酸的检测效率,可运用于实验和生产中。
A more efficient, more rapid and more sensitive ion chromatography detection program was established to detect taurine in experiment and production. The conditions were as follows：amino acid analysis column （ AminoPac PA-10,2 mm×250 mm） ,amino acid protection column （ AminoPac PA-10, 2 mm×50 mm）,mobile phase （the mobile phase A was pure water,the mobile phase B was 250 mmol/L NaOH solution,the mobile phase C was 1.0 mmol/L sodium acetate （NaOAc））,gradient elution,flow rate （ 0.24 mL/min ） , column temperature （ 30 ℃） , integrated pulsed amperometric detection with injection volume of 25 μL. We have successfully established the gradient elution program in ion chromatography to separate taurine. The present ion chromatography method for detection taurine was efficient,rapid,accurate and the detection time was greatly improved.
出处
《生物加工过程》
CAS
2015年第3期64-69,共6页
Chinese Journal of Bioprocess Engineering
基金
国家自然科学基金(21406185)
关键词
牛磺酸
离子色谱
稳定性
精确度
回收率
taurine
ion chromatography
stability
precision
recovery
