摘要
目的：探讨人乳头状瘤病毒L1壳蛋白和人端粒酶RNA基因联合检测在宫颈癌筛查中的意义。方法选取2012年10月～2014年10月新疆医科大学第一附属医院行宫颈癌筛查的患者627例，先行液基细胞学检查，后行阴道镜检查并取活检，同时行人乳头状瘤病毒L1壳蛋白检测和人端粒酶RNA基因检测。分析不同病症间临床指标的变异情况。结果未见上皮病变或恶性改变（NILM）、意义不明的不典型鳞状细胞与不典型腺细胞（ASC-US）、不除外高度鳞状上皮内病变的的不典型鳞状细胞（ASC-H）、低度鳞状上皮内病变（LSIL）、高度鳞状上皮内病变（HSIL）、鳞状细胞癌（SCC）、腺癌（AC），人乳头状瘤病毒L1壳蛋白阳性表达率分别为18.6%、42.9%、51.0%、80.6%、47.4%、5.9%、0.0%，差异有高度统计学意义（χ2=93.770，P〈0.01），呈先升高后降低趋势，在LSIL达到最高点，在AC降至最低点；人端粒酶RNA基因阳性表达率分别为0.0%、19.9%、36.3%、51.6%、94.7%、100.0%、100.0%，差异有高度统计学意义（χ2=279.564，P〈0.01），呈持续升高趋势，在SCC与AC达到最高点。依正常、炎症、宫颈上皮内瘤变（CIN）Ⅰ期、CINⅡ期、CINⅢ期、宫颈癌顺序，人乳头状瘤病毒L1壳蛋白阳性表达率分别为18.6%、46.1%、80.6%、53.3%、25.0%、3.6%，差异有高度统计学意义（χ2=93.015，P〈0.01），呈先升高后降低趋势，在CINⅠ期达到最高点，在宫颈癌降至最低点；人端粒酶RNA基因阳性表达率分别为0.0%、26.4%、51.6%、93.3%、100.0%、100.0%，差异有高度统计学意义（χ2=269.582，P〈0.01），呈持续升高趋势，在CINⅢ期达到最高点。结论人乳头状瘤病毒L1壳蛋白联合人端粒酶RNA基因检测，在宫颈癌前病变中具有较高的指导价值。
Objective To investigate significance of human papilloma virus L1 protein and human telomerase RNA gene combined detection in cervical cancer screening. Methods From October 2012 to October 2014, in the First Af-filiated Hospital of Xinjiang Medical University, 627 patients given cervical cancer screening were selected. Firstly, the patients received liquid based cytology, then they received colposcopy and biopsy, at the same time, they also received human papilloma virus L1 protein detection and human telomerase RNA gene detection. The variation of clinical indi-cators were analyzed between different conditions. Results According to sequence of NILM, ASC-US, ASC-H, LSIL, HSIL, SCC, AC, positive expression ratio of human papilloma virus L1 protein were 18.6%, 42.9%, 51.0%, 80.6%, 47.4%, 5.9%, 0.0%, the difference was statistically significant （χ2=93.770, P〈0.01）, which showed increasing at first and then decreasing, it reached highest point at LSIL , and reached lowest point at AC; positive expression ratio of hu-man telomerase RNA gene were 0.0%, 19.9%, 36.3%, 51.6%, 94.7%, 100.0%, 100.0%, the difference was statistically significant （χ2= 279.564, P〈 0.01）, which showed continue increasing, it reached highest point at SCC and AC. Ac-cording to sequence of normal, inflammation, CIN phase Ⅰ, CIN phase II, CIN phase Ⅲ, cervical cancer, positive ex-pression ratio of human papilloma virus L1 protein were 18.6%, 46.1%, 80.6%, 53.3%, 25.0%, 3.6%, the difference was statistically significant （χ2 = 93.015, P〈 0.01）, which showed increasing at first and then decreasing, it reached highest point at CIN phase Ⅰ, and it reached lowest point at cervical cancer; positive expression ratio of human telomerase RNA gene were 0.0%, 26.4%, 51.6%, 93.3%, 100.0%, 100.0%, the difference was statistically significant （χ2 = 269.582, P 〈 0.01）, which showed continue increasing, it reached highest point at CIN phase II. Conclusion Human papilloma virus L1 protein and human telomerase RNA gene combined detection has higher guiding value in cervical precancerous lesions.
出处
《中国医药导报》
CAS
2015年第17期60-63,共4页
China Medical Herald
