摘要
背景:有研究表明骨形态发生蛋白9能促进多种干细胞的成骨分化,但其是否具有诱导牙囊细胞成骨向分化的能力尚不清楚。目的:探讨骨形态发生蛋白9对大鼠牙囊细胞成骨分化的诱导作用。方法:以纯化的第3代大鼠牙囊细胞为研究对象,感染骨形态发生蛋白9腺病毒后,检测牙囊细胞中碱性磷酸酶活性、钙盐沉积以及矿化相关因子基因和蛋白的表达变化。结果与结论:感染骨形态发生蛋白9的牙囊细胞碱性磷酸酶活性持续增强,钙盐沉积明显增强。Real time PCR检测结果显示感染骨形态发生蛋白9的牙囊细胞中矿化相关因子碱性磷酸酶、骨钙素、骨涎蛋白、骨桥蛋白和核心结合因子mR NA表达增强。Western blot检测结果显示感染骨形态发生蛋白9的牙囊细胞中骨桥蛋白的表达增强。以上结果表明骨形态发生蛋白9可诱导牙囊细胞向成骨方向分化。
BACKGROUND：Bone morphogenetic protein 9 is proved to promote the osteogenic differentiation of various kinds of stem cel s, but whether it can induce the osteogenic differentiation of dental fol icle cel s in vitro is yet unclear. OBJECTIVE：To investigate whether bone morphogenetic protein 9 can induce the osteogenic differentiation of rat dental fol icle cel s in vitro. METHODS：Purified rat dental fol icle cel s at passage 3 were transfected with bone morphogenetic protein 9 adenovirus. Then, alkaline phosphatase activity, calcium deposition and expression of osteogenesis-related factors at mRNA and protein levels were detected in the dental fol icle cel s. RESULTS AND CONCLUSION：After transfection with bone morphogenetic protein 9, the dental fol icle cel s showed continuously enhanced alkaline phosphatase activities and obviously enhanced calcium deposition. Real-time PCR results demonstrated that the mRNA expressions of alkaline phosphatase, osteocalcin, bone sialoprotein, osteopontin and core binding factor were increased significantly. The western blot assay showed that the expression of osteopontin enhanced in the dental fol icle cel s after transfection with bone morphogenetic protein 9. In summary, bone morphogenetic protein 9 can induce the osteogenic differentiation of dental fol icle cel s.
出处
《中国组织工程研究》
CAS
北大核心
2015年第14期2255-2260,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金青年基金资助项目(81300914)
2013年重庆高校创新团队建设计划资助项目
重庆市高校市级口腔生物医学工程重点实验室资助项目~~
