摘要
目的:观察孕酮(PROG)对大鼠局灶性脑缺血/再灌注损伤后的神经保护作用,并探讨其作用机制。方法:120只雄性SD大鼠随机分为:假手术组、大脑中动脉栓塞(MCAO)组和PROG+MCAO组(n=40)。线栓法建立大鼠右侧MCAO模型,PROG+MCAO组于建模型前30 min按8 mg/kg腹腔内注射PROG。大鼠脑缺血2 h再灌注0、24、48、72 h,通过Longa评分标准进行神经功能缺陷评分;实时荧光定量聚合酶链反应技术检测大鼠脑组织中双孔道结构域钾离子通道3(TASK3)mRNA的表达。结果:PROG(8 mg/kg)可显著降低大鼠脑缺血2 h再灌注24、48、72 h时的神经功能缺陷评分(P<0.05)。与假手术组相比,MCAO组再灌注各时间点脑组织TASK3 mRNA的表达均显著降低(P<0.05);与MCAO组相比,PROG+MCAO组再灌注各时间点大鼠脑组织中TASK3 mRNA的表达均显著增多(P<0.05)。结论:PROG可改善局灶性脑缺血/再灌注损伤后大鼠神经功能缺陷症状,其作用机制可能与上调脑组织中TASK3 mRNA的表达有关。
Objective： To observe the neurological protective effects of progesterone （ PROG ） on focal cerebral ischemia/reperfusion in- jury in rats and to explore its possible mechanism. Methods： One handred and twenty male SD rats were divided into three groups randomly： sham-operated group, middle cerebral artery occlusion （ MCAO ） group and PROG ＋ MCAO group（ n = 40）. The right temporary MCAO mode/was established by the line-embolism method. The PROG ＋ MCAO group rats were according to 8 mg/kg intraperitoneal injection PROG , after that 30 min, the rats were suffered ischemia/reperfusion. After rats were suffered ischemia for 2 h and reperfusion 0, 24, 48, 72 h stress, the nervous functional defect degree were evaluated by （TASK3） mRNA in brain tissue were detected by the real-time PCR. longe scoring, and the expression of two-pore domain K channel 3 Results： PROG （ 8 mg/kg ） could significantly reduced the nervous functional defect degree in rats after ischemia/reperfusion 24, 48, 72 h （ P 〈 0.05）. The results of real-time PCR showed that the TASK3 mRNA expression in the brain tissue at all time points significantly decreased in MCAO group compared with sham-operated group （ P 〈 0. 05）. However, compared with MCAO group, the expression of TASK3 mRNA in brain tissue at all time points dramatically increased in PROG ＋ MCAO group （ P 〈 0.05）. Conclusion： PROG can improve the nervous functional defect degree after focal cerebral ischemia/reperfusion injury in rats, and the mechanism might be associated with up-regulating the expression of TASK3 mRNA in brain tissue.
出处
《中国应用生理学杂志》
CAS
CSCD
2015年第3期231-234,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助项目(81100912
81271376)
关键词
孕酮
脑缺血
再灌注损伤
TASK3
progesterone
cerebral ischemia
ischemia/reperfusion injury
TASK3
