摘要
目的:研究丹皮酚脂质体中丹皮酚、磷脂和胆固醇三者分子间相互作用。方法:采用示差扫描量热(DSC)和同步辐射X光衍射(XRD)两种方法研究了丹皮酚脂质体中丹皮酚、胆固醇和二棕榈酰磷脂酰胆碱(DPPC)分子间的相互作用。结果:DSC实验结果表明,4%的丹皮酚或者胆固醇都使DPPC脂质体的前相变消失,主相变温度从42℃降低到38℃,二者都使DPPC脂质体主相变的协同性明显下降,但丹皮酚使DPPC脂质体主相变协同性下降更多。同步辐射X光衍射峰的峰形分析表明,4%的丹皮酚或者胆固醇都使脂质体发生明显的分相现象,且丹皮酚诱导的分相现象更加明显,与DSC实验结果一致。结论:DSC和XRD实验结果都表明,丹皮酚脂质体中胆固醇与DPPC疏水链的分子间相互作用要大于丹皮酚与DPPC疏水链的分子间相互作用,预示着在确定丹皮酚脂质体的处方时胆固醇的浓度不宜过大,否则将影响丹皮酚的包封率。
Objective：To investigate molecular interaction among paeonol, cholesterol and phospholipid in pae-onol-encapsulated liposomes.Methods：Differential scanning calorimetry （ DSC） and synchrotron x-ray dif-fraction （ XRD） techniques were employed to investigate molecular interaction among paeonol, cholesterol and dipalmitoyl-phosphatidylcholine （ DPPC） in paeonol-encapsulated liposomes.Results：DSC data show that the pretransition of DPPC liposomes containing 4 %paeonol or cholesterol disappeared and the temperature of main transition decreased from 42℃to 38℃.Though the cooperativity of main transition of DPPC liposomes containing 4 %paeonol or cholesterol both decreased obviously, it was paeonol that caused a smaller cooperat-ivity.XRD data showed that phase separation appeared in the DPPC liposomes containing 4%paeonol or cho-lesterol and it was paeonol that caused a more obvious phase separation, which was in accordance with DSC data.Conclusion：Both DSC data and XRD data show that the molecular interaction between cholesterol and hydrocarbon chains of DPPC is stronger than that between paeonol and hydrocarbon chains of DPPC.This re-sult suggests that the high concentration of cholesterol in paeonol-encapsulated liposomes can suppress the entrapment efficiency of paeonol.
出处
《中医药信息》
2015年第3期14-16,共3页
Information on Traditional Chinese Medicine
基金
北京市自然科学基金资助项目(No.7153171)
