摘要
以高丛康维尔蓝莓组培苗为材料,用不同质量浓度的秋水仙素通过浸渍法和培养法进行染色体加倍研究,从形态学、生理生化学、解剖学及细胞学进行比较,以确定能够诱导出多倍体的最佳处理方法和处理质量浓度.结果发现,在形态指标上,0.5,2.0,4.0 mg/m L秋水仙素浸渍处理1 d及0.4 mg/m L秋水仙素培养10 d后,蓝莓的叶长、叶宽、茎直径、叶厚、茎直径的平均值均显著大于CK;在解剖结构上,0.5 mg/m L浸渍1 d和0.4 mg/m L培养10 d后,其气孔变大,密度变低,为椭圆形,保卫细胞的淀粉粒减少,0.5,2.0 mg/m L秋水仙素浸渍1 d和0.4 mg/m L培养10 d后均使细胞核明显变大;在生理指标上,0.5 mg/m L浸渍处理1 d后显著提高了蓝莓SOD酶活性,2.0,4.0 mg/m L秋水仙素浸渍处理1 d能够显著提高可溶性蛋白质含量,其中后者还显著提高了其叶绿素A含量.可见,0.5 mg/m L浸渍1 d和0.4 mg/m L培养10 d后,蓝莓植株发生多倍性变异的可能性最大,但还需后续的大田鉴定,才能最终确定多倍体植株.
Taking High-bush Convair blueberry plantlets as materials,through impregnation and culture with colchicine of different concentrations,chromosome doubling was studied.From the morphology,physiology and biochemistry,anatomy and cytology were compared in order to determine the best treatment method and treatment quality concentration to induce polyploidy.The results showed that,in morphology and anatomy,the mean of blueberry the leaf length,leaf width,stem diameter and leaf thickness were significantly greater than CK after 0.5,2.0 and 4.0 mg/mL colchicine soaking for 1 d and 0.4 mg/mL colchicine culture for 10 d;in anatomical structure,0.5 mg/mL impregnated for 1 d and 0.4 mg/mL culture for 10 d,the stomatal density of blueberry becomes larger,low, oval,starch grains in guard cells reduced,and the nucleus becomes larger after 0.5,2.0 mg/mL soaking for 1 d and 0.4 mg/mL colchicine impregnated culture for 10 d were significantly larger nuclear;in the physiological indexes,0.5 mg/mL impregnated for 1 d significantly increased SOD activity of blueberry,colchicine soaking for 1 d and 4.0 mg/mL could significantly improve soluble protein content,the latter also significantly increased chlorophyll a content.Thus,after 10 d culture 0.5 mg/mL impregnated for 1 d and 0.4 mg/mL,the possibility of the occurrence of the blueberry plant polyploidy variant of the maximum,but need field identification of follow-up to ultimately determine the polyploid plants.
出处
《昆明学院学报》
2015年第3期72-76,共5页
Journal of Kunming University
关键词
蓝莓
秋水仙碱
诱变
多倍体
鉴定
blueberry
colchicine
mutagenesis
polyploidy
identification
