摘要
目的通过酶联免疫吸附试验（enzyme-linked immunosorbent assay,ELISA）检测增殖性糖尿病视网膜病变（proliferative diabetic retinopathy,PDR）患者血清中的Dickkopf-1（DKK1）蛋白浓度,探讨Wnt信号通路在PDR发病中的作用。方法纳入2014年6月至12月于北京安贞医院及北京同仁医院眼科接受玻璃体视网膜手术的28例PDR患者及25例年龄、性别相匹配的非糖尿病对照组。通过ELISA法检测PDR组和对照组、活跃期和静止期PDR组血清中的DKK1蛋白浓度,分别比较组间的DKK1水平差异。结果 PDR组血清DKK1蛋白浓度为96.14~1303.65 pg·mL-1,平均为491.79 pg·mL-1;对照组血清DKK1蛋白浓度为250.36~1520.92 pg·mL-1,平均为882.38 pg·mL-1;两组相比,差异有统计学意义（P=0.002）。静止期PDR组血清DKK1蛋白平均浓度为683.35 pg·mL-1,高于活跃期PDR组（275.48 pg·mL-1）,差异有统计学意义（P=0.022）。结论 PDR患者血清中DKK1蛋白水平明显降低,提示Wnt信号通路可能在PDR病理过程中发挥作用。
Objective To examine Dickkopf-1 （DKK1） levels in the serum of pa- tients with proliferative diabetic retinopathy （PDR） by enzyme-linked immunosorbent assay （ELISA） ,and evaluate the role of Wnt signaling pathway in the development of PDR. Methods Twenty-eight cases with PDR received pars plana vitrectomy in Bei- jing Anzhen Hospital and Beijing Tongren Hospital from June 2014 to December 2014 were prospectively recruited. Another 25 non-diabetic mellitus control cases were matched with age and gender. DKK1 levels in the serum of PDR group （ active PDR,qui- escent PDR） and controls were measured by ELISA. Mann-Whitney U test was used to analyze the differences between two groups. Results Serum levels of DKK1 in PDR group was 491.79 pg · mL-1 （96.14 - 1303.65）pg · mL-1 ,which in non-diabetic mellitus group was 882.38 pg · mL-1 （250.36 - 1520. 92）pg · mL-1 ,the PDR group was lower than the non-diabetic mellitus group （P = 0. 002 ）. Furthermore, the serum concentra- tions of DKK1 in quiescent PDR group （683.35 pg · mL-1 ） was higher than that in ac- tive PDR group （275.48pg· mL-1）（P=0.022）.Conelusion DKKI levels in the ser- um of patients with PDR are significantly decreased,indicating the participation of Wnt signaling pathway in the pathological processes of PDR.
出处
《眼科新进展》
CAS
北大核心
2015年第6期536-538,共3页
Recent Advances in Ophthalmology
基金
国家自然科学基金资助(编号:30872820
81271034)~~
