摘要
禽致病性大肠杆菌的毒力因子在其致病过程中发挥了重要作用,目前研究较多的毒力因子包括:菌毛、非菌毛黏附素、耶尔森菌强毒力岛(HPI)、溶血素、抗血清存活因子、毒素、侵袭素等。为了解不同毒力基因在菌株间分布规律,选定7个主要毒力因子的编码基因:fimC(Ⅰ型菌毛)、kpsM(荚膜多糖)、cvaC(大肠杆菌素)、sodA(超氧化物歧化酶)、csgA(curli菌毛)、tsh(温度敏感性血凝素)和marA(多重耐药调控基因),通过对本实验室保存的34株不同禽源的禽致病性大肠杆菌进行毒力基因的检测,结果发现,fimC(94.1%)、cvaC(82.4%)、sodA(97.1%)、csgA(97.1%)、tsh(76.5%)和marA(100%)这6个毒力基因的分布率均较高,但相互之间也存在差异。kpsM基因的分布率较低,只有0.09%,并且仅在鸡源性禽致病性大肠杆菌中检测到。
Virulence factors of avian pathogenic Escherichia coli play an important role in the pathogenic process where some major virulence factors in this bacteria include,such as fimbria,afimbrial adhesin,Yersinia high-pathogenicity island（HPI）,hemolysin,antiserum survival factor,toxins and invasins. To study the distribution rule of major virulence genes,seven main virulence factor genes encoding：fimC（typeⅠfimbriae）,kpsM（capsular polysaccharide）,cvaC（colicin）,sodA（superoxide dismutase）,csgA（curli）and tsh（hemagglutinin temperature sensitivity）and marA（multiple drug-resistant genes）were selected to detect in 34 isolates of different origins. The results found that the distribution of fimC（94.1%）,cvaC（82.4%）and sodA（97.1%）,csgA（97.1%）,tsh（76.5%）and marA（100%）were higher than other genes,but there were differences among them. The distribution rate of kpsM gene was low at only 0.09%,and avian pathogenic E. coli with this gene was detected only in the chicken origin.
出处
《中国家禽》
北大核心
2015年第10期34-37,共4页
China Poultry
基金
江苏高校优势学科建设工程
国家自然科学基金(30571374
30771603
31072136
31270171)
