摘要
为巨菌草的种质鉴定、遗传育种和亲缘关系提供一定的细胞学基础,采用传统压片法,对象草（Pennisetum purpureum schumach）和巨菌草〔Pennisetum giganteum z.x.lin（暂定名）〕的染色体数目及核型进行分析。结果表明：象草和巨菌草的染色体形态较为一致,大部分由中部（m）和近中部（sm）着丝粒的染色体组成,象草和巨菌草的核型公式分别为2n=4x=28=20m＋8sm和2n=4x=28=16m＋8sm＋4st;染色体相对长度组成分别为2n=4x=28=6L＋6m^2＋10M1＋6S和2n=4x=28=4L＋8m^2＋10 M1＋6S,染色体基数和总数目一致,核型分类均为2A型,不对称系数为58%-60%,均属于较原始的类型。结论：象草和巨菌草的的染色体无明显差异性,从细胞染色体水平上认为两者亲缘关系较近。
To provide certain cellular basis for germplasm, breeding and genetic kinship of P .giganteum,The karyotype analysis and chromosome number of P .purpureum and P .giganteum were conducted by traditional squash method.The results showed that the karyotype morphology of materials were similar,consisted of metacentric chromosomes and sub-metacentric chromosomes mostly.In this study, the karyotype formulas of P . purpureum and P . giganteum were respectively 2 n=4 x=28=20 m＋8 sm,2 n=4 x=28= 16 m＋8 sm＋4 st.Constitutions of relative length of two plants were 2 n=4 x=28=6 L＋6 m^2 ＋ 10 M1 ＋6 S,2 n=4 L＋8 m^2 ＋ 10 M1 ＋ 6S.The karyotype of P .purpureum was the same as that of P .giganteum in chromosome basic number and total number.And two varieties were classed as 2A.The asymmetry coefficient were in the range of 58% to 60%,which demonstrated basic symmetry karyotypes. Conclusion： Both of the two plants had no significant differences in chromosomes between them and the relationship between them was close from cellular level.
出处
《贵州农业科学》
CAS
2015年第5期14-18,共5页
Guizhou Agricultural Sciences
基金
福建省科技重大专项"水土流失初步治理区生态循环与产业提升技术研发与示范"(2012NZ0002)
科技部项目"国家菌草工程技术研究中心组建"(2011FU125X12)
关键词
象草
巨菌草
染色体
核型
Pennisetum purpureum
Pennisetum giganteum
chromosome
karyotype
