摘要
以多花勾儿茶果实、叶及自制绿茶为原料,采用水浴法提取儿茶素,研究其最佳提取工艺.提取液经石油醚脱脂溶性色素后,采用大孔吸附树脂D941进行吸附分离,静态吸附和解析条件研究得出纯净水洗脱效果较好.多花勾儿茶果实、叶及自制绿茶吸附分离的纯化液,用色谱柱:Boston Green ODS PC18(4.6×250mm,5μm),检测波长279nm,流动相乙腈-0.4%磷酸(w(乙腈)∶w(0.4%磷酸)=13∶87),柱温35℃,流速1 mL/min,一次进样10μL来进行高效液相色谱(HPLC)检测,其结果显示,多花勾儿茶果实中儿茶素含量最低,自制绿茶中的儿茶素含量最高.
In this study ,the extraction process of catechins was investigated with a certain concentration of ethanol as solvent from man‐made green tea ,fruits and leaves of Berchemia floribunda ,using the method of waterbath .After the fat soluble pigment was removed by petroleum ether from extracting solution ,the catechins was purified by porous resin adsorption method .The static adsorption and desorption tests of catechin with macroporous resin showed that D941 was the optimal resin for purification and the pure water was the optimum eluant .Purified liquid of fruit ,leaves and home‐made green tea extracts were de‐tected by HPLC .The catechins was separated on PC18 chromatographic column （4 .6 x 250 mm ,5 μm） at 35 ℃ ,with nitrile‐0 .4% phosphoric acid （13∶87） as mobile phase at a flow rate of 1 mL/min ,and de‐tected at the wavelength of 279 nm （UV scan） .The results showed that the catechin content of fruit was the lowest ,while the content of man‐made green tea was significantly higher than that of both the fruit and leaves through the optimized extraction processes .
出处
《吉首大学学报（自然科学版）》
CAS
2015年第3期82-89,共8页
Journal of Jishou University(Natural Sciences Edition)
基金
湖南省科技计划资助项目(2013JT2012)
张家界市科技计划资助项目(2014ZK01)
生态旅游湖南省重点实验室开放基金资助项目(JDSTLY1403)
林产化工工程湖南省重点实验室开放基金资助项目(JDZ201404)
