摘要
目的阐述K9(C4H4FN2O2)2Ce(PW11O39)2·23H2O(FNd PW)诱导卵巢癌细胞SKOV3凋亡作用的机制。方法 MTT法检测细胞生长;吖啶橙/溴乙锭(AO/EB)与电镜检测细胞形态;半胱氨酸的天冬氨酸蛋白水解酶(Caspase-3)活性试剂盒检测Casapse-3的变化;Western Blot法检测细胞中B淋巴细胞瘤-2(Bcl-2)、多肽抗原(Bax)以及细胞色素C蛋白的表达。结果 FNd PW降低了卵巢癌SKOV3的细胞活力,IC50=3.56μmol/L。FNd PW能够使Bcl-2蛋白表达下调,促凋亡蛋白Bax表达上调,能够增加Caspase-3的活性,同时使细胞色素C表达上调。结论 FNd PW通过促进卵巢癌细胞系SKOV3的凋亡,从而引起细胞增殖下降,导致细胞死亡。为FNd PW可成为临床卵巢癌患者的有效治疗药物提供了实验依据。
Objective To illustrate the mechanism of K9（C4H4FN2O2）2Ce（PWuO39）2·23H2O （FNdPW） for inducing SKOV3 apoptosis in ovarian cancer. Methods Cell growth was detected by MTT method, and cellular morphology was detected by acridine orange/ethidium bromide （AO/EB） and electron microscope. Cysteinyl aspartate specific proteinase （Caspase-3） active kit was applied for detecting Caspase-3 changes, and Western Blot method was applied in detection of expressions of B-cell lymphoma-2 （Bcl-2） and Bcl-2 Assaciated X protein （Bax） in cell and cytochrome C protein. Results FNdPW decreased viability of SKOV3 in ovarian cancer, IC50=3.56 μmol/L. FNdPW had capacity for decreasing expression of Bcl-2 protein, and increasing expression of apoptin Bax and cytochrome C, along with viability of Caspase-3. Conclusion Necrocytosis is induced by FNdPW through its accelerating SKOV3 apoptosis in ovarian cancer for decreased cell proliferation. That provides experimental reference for applying FNdPW for effective drug in clinical treatment of ovarian cancer patients.
出处
《中国现代药物应用》
2015年第13期271-273,共3页
Chinese Journal of Modern Drug Application
