摘要
啤酒糟经预处理后可以改变其紧密度以及粒径大小,从而影响酶解效率,随着粉碎程度的增加,酶解效率增加,其中,啤酒糟原样经木聚糖酶和纤维素酶水解后,阿魏酰低聚糖(FOs)的得率分别为31.89%和33.41%,木聚糖酶和纤维素酶协同作用后,FOs的得率为47.01%;刀片粉碎后球磨30 min的啤酒糟,经木聚糖酶和纤维素酶水解后,FOs的得率分别为39.49%和50.36%,而经木聚糖酶和纤维素酶协同作用后,FOs的得率增加到了64.00%。实验结果表明,预处理可以提高酶解效率,纤维素酶和β-葡聚糖酶的存在有利于木聚糖酶的作用,从而在一定程度上提高从啤酒糟中提取FOs的效率,初步实现啤酒糟的高值化利用。
Mechanical pretreatment of brewer＇ s spent grain （BSG） can change its tightness and the particle size, thus affecting enzymatic hydrolysis efficiency. With the increase of degree of crushing, enzymatic efficiency in- crease. The yield of feruloyl oligosaccharides （FOs） from untreated BSG by xylanase and cellulase were 31.89% and 33.41% , respectively. And after the synergy of xylanase and cellulase, the yield of FOs increased to 47.01%. The yield of FOs from mechanical treated BSG hydrolyzed by xylanase and cellulase were 39.49% and 50.36% , respec- tively. And after the synergy of xylanase and cellulase, the yield of FOs increased to 64.00%. The results indicated that pretreatment could improve the＂ efficiency of enzymatic hydrolysis, and the presence of cellulase and β-glucanase was beneficial to the effect of xylanase, thus improved the extraction efficiency of FOs from BSG and initially realized the high-value utilization of BSG.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第1期1-6,共6页
Food and Fermentation Industries
基金
国家自然科学基金项目(31171736)
国家863计划(2013AA102109)
江苏省普通高等学校科研成果产业化推进项目(JHB2012-26)
江苏省科技支撑计划(BE2012397)
江苏高校优势学科建设工程资助项目
江南大学糖化学与生物技术教育部重点实验室开放课题(KLCCB-KF201407)
关键词
啤酒糟
刀片粉碎
球磨
酶解
阿魏酰低聚糖
brewer＇s spent grain
blade grinding
ball milling
enzymatic hydrolysis
fernloyl oligosaccharides
