摘要
采用超声波辅助浸提油茶叶中的黄酮,优化其提取工艺,并研究其抗氧化活性。结果表明,优化后的提取工艺条件为:以50%乙醇溶液为提取剂,料液比1︰20(m︰V),提取温度70℃,超声功率300W下提取2次,每次30min。该条件下总黄酮得率为47.01 mg RE/g。油茶叶黄酮具有很强的DPPH·、ABTS+及·OH清除活性,IC50分别为15.12,21.15,410.00μg RE/mL。
Technology of ultrasonic-assisted method was optimized to extract flavonoids from leaves of camellia oleifera Abel,and antioxidant activity of flavonoids was investigated.It showed that the optimized conditions were 50% ethanol of extracting solvent,1∶20of material-liquid ratio（m ︰ V）,70 ℃ of extracting temperature,2times under 300 Wof electric power,30 min of ultrasonic treatment time,The optimized extraction yield of total flavonoids was 47.01 mg RE/g.The extraction of flavonoids from leaves of Camellia Oleifera Abel.showed strong free-radical scavenging activities of DPPH,ABTS and hydroxyl with 15.12,21.15 and 410μg RE/mL as IC50,respectively.
出处
《食品与机械》
CSCD
北大核心
2015年第3期162-166,共5页
Food and Machinery
基金
国家自科基金项目(编号:31070612/C161103)
关键词
油茶叶
总黄酮
超声辅助提取
抗氧化活性
leaves of camellia oleifera Abel.
total flavonoids
extracting technology of ultrasonic-assisted method
antioxidant activity
