摘要
【目的】探讨姜黄素对急性心肌缺血-再灌注损伤大鼠内皮素-1的影响。【方法】SD大鼠48只,随机分6组:假手术组(C)、缺血组(I)、缺血-再灌注1h组(R1h)、缺血-再灌注2h组(R2h)、姜黄素+缺血-再灌注1h组(Cur+I30min+R1h)和姜黄素+缺血-再灌注2h组(Cur+I30min+R2h)。酶联免疫法测量内皮素(ET-1)血清学水平;RT-PCR、Western blotting检测(MI/R)各组心肌组织中ET-1的变化,及Cur对ET-1表达的影响。【结果】与C组比较,I、R组血清ET-1明显升高(P<0.05);与I组比较,R组血清ET-1升高显著(P<0.05);Cur+R1、2h组血清ET-1较R1、2h明显下降(P<0.05)。与C组比较,I组ET-1mRNA表达无差异,ET-1蛋白水平升高(P<0.05);R组ET-1mRNA和蛋白表达随时间延长逐渐升高(P<0.05);Cur+R1、2h组ET-1mRNA和蛋白均较R1、2h组下降(P<0.05)。【结论】心肌缺血-再灌注损伤可试用姜黄素进行干预降低ET-1的表达。
[ Objective ] To investigate the effects of curcumin （Cur） on endothelin-1 （ET-1） in acute myocardial ischemia-reperfusion （MI/R） injury rats. [ Methods] Forty-eight SD rats were randomized into 6 groups： sham group （C）, isehemia 30 minutes group （I）, isch- emia 30 minutes ＋ reperfusion 1 hour group （Rlh）, ischemia 30 minutes ＋ reperfusion 2 hours group （R2h）, Curcumin ＋ ischemia 30 minutes ＋ reperfusion 1 hour group （Cur＋I 30 min＋Rlh）, Curcumin ＋ ischemia 30 minutes ＋ reperfusion 2 hours group （Cur＋I30min＋ R2h）. The levels of serum ET-1 were determined by ELISA and the changes of myocardial ET-1 were measured by RT-PCR and West- ern blotting methods. [Results]Compared with C group, serum ET-1 of I and R groups rose obviously （P〈0.05）. Compared with I group, serum ET-1 of R group rose significantly （P〈0.05）. Compared with Rlh and R2h groups, serum ET-1 of Cur＋I 30 min＋Rlh and Cur＋I 30 min＋R2h groups decreased significantly （P〈0.05）. Compared with C group, ET-1 mRNA expression of I group showed no sig- nificant difference, while ET-1 protein level increased （P〈0.05）. ET-1 mRNA and protein expressions of R group increased gradually as the time was prolonged（P〈0.05）. ET-1 mRNA and protein expressions of Cur＋I 30 min＋R l h and Cur＋I 30 min＋R2h groups were lower than those of R l h and R2h groups （P〈0.05）. [ Conclusion ] Curcumin can be tried to reduce ET-1 expression in myocardial ischemia- reperfusion injury..
出处
《武警后勤学院学报（医学版）》
CAS
2015年第5期342-344,共3页
Journal of Logistics University of PAP（Medical Sciences）
