摘要
目的：建立同时测定蒙药复方肉豆蔻五味中2种木脂素类成分[去氢二异丁香油酚和2,3-二氢-7-甲氧基-2-（3,4-亚甲二氧基苯基）-3-甲基-5-（E）-丙烯基-苯并二氢呋喃（简称为：木脂素类成分2号）]含量的方法。方法：采用高效液相色谱法。色谱柱为Elite C18,流动相为水-甲醇（梯度洗脱）,流速为1.0 ml/min,柱温为30℃,检测波长为225 nm,进样量为20μl。结果：去氢二异丁香油酚、木脂素类成分2号进样量分别在0.202~2.02、0.204~2.04μg范围内与其峰面积呈良好的线性关系（r=0.999 9、0.999 9）;精密度、稳定性、重复性试验的RSD〈2%;平均加样回收率分别为101.54%、99.43%,RSD分别为0.60%、1.09%（n=6）。结论：该方法简便、灵敏、准确,可用于蒙药复方肉豆蔻五味中去氢二异丁香油酚和木脂素类成分2号的含量测定。
OBJECTIVE：To establish a method for the determining contents of 2 lignan components[dehydrodiisoeugenol and2,3-dihydro-7-methoxy-2-（3,4-methylened ioxyphenyl）-3-methyl-5-（E）-propenyl-benzofuran（referred to＂lignanoid 2＂）]. METHODS：HPLC method was adopted. The column was Elite C18 with the mobile phase of water-methanol（gradient elution）at the flow rate of 1.0 ml/min;the detection wavelength was 225 nm and the column temperature was 30 ℃. The sample size was 20 μl. RESULTS：There was a good linear relationship between sample quantity and the peak area in the range of 0.202-2.02 μg（r=0.999 9）and 0.204-2.04 μg（r=0.999 9）for 2 lignan components. The RSD of precision,stability and repeatability tests were less than 2%with the average recovery of 101.54%（RSD=0.60%,n=6）and 99.43%（RSD=1.09%,n=6）. CONCLUSIONS：The method is simple,sensitive and accurate,and can be used for the quantization determination of dehydrodiisoeugenol and lignanoid 2 in nutmeg-5.
出处
《中国药房》
CAS
北大核心
2015年第18期2538-2541,共4页
China Pharmacy
基金
内蒙古自治区自然科学基金面上项目(No.2013JQ03)
内蒙古自治区高等学校"青年科技英才计划"项目(No.NJYT-14-A12)
