摘要
目的：建立同时测定加味逍遥丸中芍药苷与甘草苷含量的方法。方法：采用高效液相色谱法。色谱柱为Hypersil-ODS C18,流动相为乙腈-水（14∶86,V/V）,流速为1.0 ml/min,检测波长为230 nm,柱温为室温,进样量为10μl。结果：芍药苷、甘草苷质量浓度分别在16.12~80.6、6.08~30.4μg/ml范围内与各自峰面积呈良好的线性关系（r为0.999 6、0.999 8）;精密度、稳定性、重复性试验的RSD〈2.0%;平均加样回收率分别为98.51%、98.08%,RSD分别为1.94%、1.29%（n=6）。结论：该方法操作简单、准确度高,可用于测定加味逍遥丸中芍药苷与甘草苷的含量。
OBJECTIVE：To establish the HPLC method for the content determination of paeoniflorin and liquoritin in Jiawei xiaoyao pills. METHODS：It was performed on Hypersil-ODS C18 with the mobile phase of acetonitrile-water（14 ∶ 86,V/V）at the flow rate of 1.0 ml/min,the detection wavelength was 230 nm,the column temperature was room temperature and the volume was 10 μl. RESULTS：The linear range was 16.12-80.6 μg/ml for paeoniflorin（r=0.999 6）and 6.08-30.4 μg/ml for liquoritin（r=0.999 8）;the RSDs of precision,repeatability and stability tests were all less than 2.0%;the average recovery was respectively98.51%（RSD=1.94%,n=6）and 98.08%（RSD=1.29%,n=6）. CONCLUSIONS：The method is simple,accurate and reliable,and can be used for the content determination of paeoniflorin and liquoritin in Jiawei xiaoyao pills.
出处
《中国药房》
CAS
北大核心
2015年第18期2571-2572,共2页
China Pharmacy
