摘要
目的探讨芦荟多糖对重度创伤失血性休克大鼠海马炎症和氧化反应的影响。方法雄性SD大鼠36只,体重250-300 g。随机分为假手术组、休克组、乳酸林格液（LR）组和芦荟多糖（AP）组,芦荟多糖（AP）组按AP用量不同分为25 mg/L（AP25）、50 mg/L（AP50）和75 mg/L（AP75）3个亚组,每组6只。于复苏后2 h,取血样2 ml,测定血浆TNF-α、IL-6的含量;断头、冰上取脑,分离海马,测定海马组织MDA含量,MPO和SOD活性。结果在T6和T7时,AP50组MAP值高于LR组（t=3.833,3.190,P〈0.05）;LR组海马组织MDA含量、MPO活性和血浆TNF-α和IL-6含量低于休克组（t=-4.132,-4.887,-5.370,-2.413,P〈0.05）,而SOD活性高于休克组（t=4.623,P〈0.05）;AP50组海马组织MDA含量、MPO活性和血浆TNF-α、IL-6含量低于LR组（t=-4.092,-4.263,-2.934,-9.311,P〈0.05）,而SOD活性高于LR组（t=2.673,P〈0.05）;LR组与AP25组、AP50组与AP75组上述指标差异无统计学意义（P〉0.05）。结论 50 mg/L和75 mg/L的芦荟多糖可明显提高平均动脉压,减轻氧自由基反应和炎症反应,从而起到脑保护作用。
Objective To investigate the effect of aloe polysaccharides on the hippocampal tissue antioxidant and immunomodulatory in severe traumatic-hemorrhagic shock rats. Methods Thirty-six healthy male SD rats（ weighing 250- 300） g were randomly divided into sham group,shock group,Ringer＇s lactate group and AP group. AP group was further divided into 3 subgroups（ AP25 25 mg / L;AP50 50 mg / L;AP75 75 mg / L） according to the doses of AP,n = 6 in each group. The animals were sacrificed at two hours after resuscitation. Blood samples were collected from the left femoral artery for detecting concentrations of tumor necrosis factor（ TNF-α）,interleukin（ IL）-6;the hippocampus were separated from brain tissues on the ice for detecting superoxide dismutase（ SOD） activity and myeloperoxidase（ MPO） activity,malondialdehyde（MDA） level. Results MAP was significantly higher at T6 and T7 in AP50 group than LR group（t = 3. 833,3. 190,P 〈0. 05）. Hippocampal tissue MDA,MPO and plasma TNF-α,IL-6 levels were significantly lower in LR group than Shock group（ t =- 4. 132,- 4. 887,- 5. 370,- 2. 413,P 〈0. 05）,while SOD activity was higher in LR group than Shock group（ t = 4. 623,P 〈0. 05）;Hippocampal tissue MDA levels,MPO activity and plasma TNF-α,IL-6 levels were significantly lower in AP50 group than LR group（ t =- 4. 092,- 4. 263,- 2. 934,- 9. 311,P 〈0. 05）,while SOD activity was higher in AP50 group than LR group（ t = 2. 673,P 〈0. 05）;There were no significant difference between LR and AP25,AP50 and AP75 groups（ P 〉0. 05）. Conclusion The resuscitation by using the doses of 50 mg / L and 75 mg / L of aloe polysaccharides cansignificantly improve the mean arterial pressure,reduce the oxidative stress response and inflammation,so as to reduce the brain injury after fluid resuscitation on severe traumatic-hemorrhagic shock in rats.
出处
《中华全科医学》
2015年第8期1220-1222,共3页
Chinese Journal of General Practice
基金
全军"十一五"计划课题项目(06MA083)
关键词
芦荟多糖
海马
休克
炎症
脂质过氧化
Aloe polysaccharide
Hippocampus
Shock
Inflammation
Lipid peroxidatio
