摘要
目的：建立高效液相色谱法（HPLC）测定拉米夫定片含量的方法。方法色谱柱为Agilent ZORBAX SB-C1柱（250 mm＆#215;4.6 mm，5μm），流动相为甲醇-0.025 mol/L的乙酸铵溶液（5：95），进样量10μL，柱温35℃，检测波长277 nm。结果拉米夫定在8.56~273.8μg/mL浓度范围内线性良好，r=0.9999；平均回收率98.9%。结论此方法结果准确、专属性强，可用于该药品的质量控制。
Objective To establish an HPLC method for the determination of lamivudine tablets.Methods An Agilent ZORBAX SB-C1 column（250 mm＆#215;4.6 mm, 5μm）was adopted. The mobile phase was the mixture of methanol and 0.025 mol/L ammonium acetate solution （5：95）. The injection volume was 10μL. The column temperature was 35℃ and the detection wavelength was 277 nm.Results This method had a good linearity in the range of 8.56-273.8μg/mL for lamivudine （r=0.9999）. The average recovery was 98.9 %.Conclusion The method is accurate, reliable and specific, which can be used for the quality control of lamivudine tablets.
出处
《食品与药品》
CAS
2015年第3期192-194,共3页
Food and Drug
