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Whole-genome identification and expression analysis of K^+ efflux antiporter(KEA) and Na^+/H^+ antiporter(NHX) families under abiotic stress in soybean 被引量:6

Whole-genome identification and expression analysis of K^+ efflux antiporter(KEA) and Na^+/H^+ antiporter(NHX) families under abiotic stress in soybean
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摘要 Sodium toxicity and potassium insufficient are important factors affecting the growth and development of soybean in saline soil. As the capacity of plants to maintain a high cytosolic, K^+/Na^+ ratio is the key determinant of tolerance under salt stress. The aims of the present study were to identify and analyse expression patterns of the soybean K^+ efflux antiporter(KEA) gene and Na^+/H^+ antiporter(NHX) gene family, and to explore their roles under abiotic stress. As a result, 12 soybean Gm KEAs genes and 10 soybean Gm NHXs genes were identified and analyzed from soybean genome. Interestingly, the novel soybean KEA gene Glyma16g32821 which encodes 11 transmembrane domains were extremely up-regulated and remained high level until 48 h in root after the excessive potassium treatment and lack of potassium treatment, respectively. The novel soybean NHX gene Glyma09g02130 which encodes 10 transmembrane domains were extremely up-regulated and remained high level until 48 h in root with Na Cl stress. Imaging of subcellular locations of the two new Glyma16g32821-GFP and Glyma09g02130-GFP fusion proteins indicated all plasma membrane localizations of the two novel soybean genes. The 3D structures indicated that the two soybean novel proteins Glyma09g02130(NHX) and Glyma16g32821(KEA) all belong to the cation/hydrogen antiporter family. Sodium toxicity and potassium insufficient are important factors affecting the growth and development of soybean in saline soil. As the capacity of plants to maintain a high cytosolic, K^+/Na^+ ratio is the key determinant of tolerance under salt stress. The aims of the present study were to identify and analyse expression patterns of the soybean K^+ efflux antiporter(KEA) gene and Na^+/H^+ antiporter(NHX) gene family, and to explore their roles under abiotic stress. As a result, 12 soybean Gm KEAs genes and 10 soybean Gm NHXs genes were identified and analyzed from soybean genome. Interestingly, the novel soybean KEA gene Glyma16g32821 which encodes 11 transmembrane domains were extremely up-regulated and remained high level until 48 h in root after the excessive potassium treatment and lack of potassium treatment, respectively. The novel soybean NHX gene Glyma09g02130 which encodes 10 transmembrane domains were extremely up-regulated and remained high level until 48 h in root with Na Cl stress. Imaging of subcellular locations of the two new Glyma16g32821-GFP and Glyma09g02130-GFP fusion proteins indicated all plasma membrane localizations of the two novel soybean genes. The 3D structures indicated that the two soybean novel proteins Glyma09g02130(NHX) and Glyma16g32821(KEA) all belong to the cation/hydrogen antiporter family.
出处 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第6期1171-1183,共13页 农业科学学报(英文版)
基金 supported by the National Natural Science Foundation of China(31401407) the Natural Science Foundation of Jiangsu Province,China(BK2012374) the Open Project of the State Key Laboratory of Crop Genetics and Germplasm Enhancement,China(ZW2013007) the Jiangsu Science and Technology Support Program,China(BE2013350)
关键词 soybean KEA NHX and Na antiporter abiotic potassium extremely transmembrane maintain soybean KEA NHX and Na antiporter abiotic potassium extremely transmembrane maintain
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  • 1Altschul S F, Madden T L, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman D J. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Research, 25, 3389-3402.
  • 2Apse M P, Aharon G S, Snedden W A, Blumwald E. 1999. Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis. Science, 285, 1256-1258.
  • 3Aranda-Sicilia M N, Cagnac O, Chanroj S, Sze H, Rodrfguez- Rosales M P, Venema K. 2012. Arabidopsis KEA2, a homolog of bacterial KefC, encodes a K+/H+ antiporter with a chloroplast transit peptide. Biochimica et Biophysica Acta, 1818, 2362-2371.
  • 4Baltierra F, Castillo M, Gamboa M C, Rothhammer M, Krauskopf E. 2013. Molecular characterization of a novel Na+/H+ antiporter cDNA from Eucalyptus globulus. Biochemical and Biophysical Research Communications, 430, 535-540.
  • 5Bassil E, Tajima H, Liang Y C, Ohto M A, Ushijima K, Nakano R, Esumi T, Coku A, Belmonte M, Blumwald E. 2011. The Arabidopsis Na+/H+ antiporters NHXl and NHX2 control vacuolar pH and K+ homeostasis to regulate growth, flower development, and reproduction. The Plant Ceil, 23, 3482-3497.
  • 6Bhandal I S, Malik C P. 1988. Potassium estimation, uptake, and its role in the physiology and metabolism of flowering plants. International Review of Cytology--A Survey of Cell Biology, 110, 205-254.
  • 7Blumwald E. 2000. Sodium transport and salt tolerance in plants. Current Opinion in Cell Biology, 12, 431-434.
  • 8Chen H T, Chen X, Gu H P, Wu B Y, Zhang H M, Yuan X X, Cui X Y. 2014. GmHKT1;4, a novel soybean gene regulating Na+/K+ ratio in roots enhances salt tolerance in transgenic plants. Plant Growth Regulation, 73, 299-308.
  • 9Chen H T, Cui S Y, Fu S X, Gai J Y, Yu D Y. 2008. Identification of quantitative trait loci associated with salt tolerance during seedling growth in soybean (Glycine max L.). Australian Journal of Agricultural Research, 59, 1086-1091.
  • 10Chen H T, He H, Yu D Y. 2011. Overexpression of a novel soybean gene modulating Na+ and K+ transport enhances salt tolerance in transgenic tobacco plants. Physiologia Plantarum, 141, 11-18.

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