摘要
目的:通过比较三黄泻心汤水、醇提取物的抗炎作用,确定有效提取物并对有效提取物进行成分分析,探讨三黄泻心汤抗炎的药效物质基础。方法:以二甲苯所致的小鼠耳肿胀为抗炎活性筛选模型,口服灌胃给药,通过计算各给药组的小鼠耳肿胀抑制率,确定三黄泻心汤的有效提取物,并运用HPLC法对有效提取物进行化学成分分析。结果:与模型组比较,各给药组肿胀度均有所下降,醇提物的大、中剂量组能够显著降低小鼠耳肿胀度(P<0.01),肿胀抑制率约46%;2种提取物相同剂量组间比较,醇提物的大、中剂量组肿胀度显著低于水提物(P<0.05),醇提物的抗炎活性优于水提物。黄酮类-生物碱类-蒽醌类成分在醇提物中的质量比约9∶2∶5。结论:三黄泻心汤醇提物具有显著的抗炎作用,是该方抗炎的有效提取物,其抗炎作用与有效提取物中化学成分的组成及各类成分的量比关系密切相关。
Objective： To determine the active extracts and uncover the material basis of Sanhuang Xiexin Tang,comparing the anti-inflammatory effect of its water and ethanol extracts. Method： Use the xyleneinduced auricular swelling of mouse as screening model： oral administration,and calculate the inhibition degree of swelling to determine the active extracts. Meanwhile,conduct the component analysis for active extracts. Result：Compared with model group,decrease of swelling in different groups is observed,the swelling of mouse is significantly lower of ethanol extracts than water extracts（ P〈0. 01） and the inhibition degree is about 46%. The swelling of mouse in the medium dose and high dose of ethanol extracts are significantly lower than water extracts（ P〈0. 05）,the inflammatory effect of ethanol extract is better than water extracts. The ratio of flavone,anthraquinone and alkaloid in ethanol extracts is 9∶2∶5. Conclusion： The inflammatory effect of ethanol extracts is obvious,which has something to do with the component ratio within the extracts. And ethanol extracts is determined as the active extracts of Sanhuang Xiexin Tang.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第13期31-35,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金面上项目(81274087)
关键词
三黄泻心汤
提取物
抗炎
成分分析
Sanhuang Xiexin Tang
extracts
anti-inflammatory
component analysis
