摘要
目的:建立非水反相色谱法测定火麻仁药材中亚油酸甘油三酯的含量。方法:伊利特Hypersil ODS2色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-异丙醇(75∶25),流速1.0 m L·min-1,检测波长205 nm,柱温25℃。结果:亚油酸甘油三酯在0.016~0.33 g·L-1(r=0.999 8)线性关系良好,平均加样回收率97.6%,RSD 1.4%。结论:该法采用非水反相色谱,结合常规紫外检测器,建立了一种准确可靠,通用性较强的甘油三酯的含量测定方法。
Objective: To establish a quantitation of triacylglycerols in Cannabis Semen by non-aqueous reversed-phase high-performance liquid chromatography. Method: The chromatography was carried out on Hypersil ODS2 column( 4. 6 mm × 250 mm,5 μm) with acetonitrile-isopropanol( 75∶ 25) as the mobile phase at a flow rate of 1. 0 m L·min- 1,the detection wavelength was set at 205 nm. Result: The linear rang of linolein was0. 016-0. 33 g·L- 1( r = 0. 999 8). The average recovery was 97. 6%,RSD 1. 4%. Conclusion: A non-aqueous reversed-phase high-performance liquid chromatography( NARP-HPLC) was established for determination triacylglycerols in Cannabis Semen.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第13期53-55,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81202857)
<中国药典>2015年版一部科研任务(火麻仁及饮片)