摘要
目的:采用虚拟组织学血管内超声技术探讨吡格列酮抗动脉粥样硬化(As)及降低血栓事件发生的作用。方法:选取14只新西兰大白兔,以高脂饲养及腹主动脉球囊拉伤的方法复制As模型。喂养12周后随机分为吡格列酮组(吡格列酮每天10mg/kg,n=6)和对照组(n=6),并对两组兔行虚拟组织学血管内超声(VH-IVUS)检查,两组各选取40个斑块,测量相关参数,继续高脂喂养12周后,再次行VH-IVUS检查。最后药物诱发斑块血栓形成,处死并取出腹主动脉观察所选斑块血栓事件发生情况。对两组VH-IVUS相关参数及血栓事件进行比较。结果:经过12周的药物干预,吡格列酮组的TG、hs-CRP、MMP-9下降,HDL-C升高。VH-IVUS分析显示和对照组相比,吡格列酮组的坏死成分绝对面积[-0.10(0.00,0.10)vs.0.10(0.00,0.13)mm2,P<0.001]、坏死成分相对比例[(-2.35±3.11)vs.(1.87±4.62)%,P=0.012];钙化成分绝对面积[0.00(0.00,0.10)vs.0.10(0.00,0.10)mm2,P<0.001]、钙化成分相对比例[0.00(-3.12,0.14)vs.2.00(-0.19,3.19)%,P=0.002]明显减少。吡格列酮组的血栓事件发生率低于对照组(P<0.001)。结论:吡格列酮可以引起动脉粥样斑块的回缩,降低斑块血栓事件的发生,是新型的抗As药物。
Objective：To assess the effect of pioglitazone on anti-atherosclerotic and reduce the rate of atherothrombosis by virtual histology intravascular ultrasound（VH-IVUS） in a rabbit model. Methods： Athero- sclerotic plaques were induced in the abdominal aorta of 14 New Zealand white rabbits by a combination of a hy- perlipidemic diet and balloon endothelial denudations. After 12 weeks, 14 rabbits randomly divided into two groups： 6 rabbits continued the same diet, whereas 6 rabbits received pioglitazone（ 10rag. kg-1. d） in addition to the diet. Two group rabbits underwent VH-IVUS at 12 weeks ,40 plaque were selected respectively. Two group rabbits continued a hyperlipidemic diet for 12 weeks, then underwent VH-IVUS again. At the end, the rabbits underwent pharmaceutical triggering to induce atherothrombosis. We make a statistical analysis of the parameter received from VI-I-IVUS and atherothrombosis. Results： In the pioglitazone group after pharmacological inter- vention of 12 weeks, plasma triglyceride, high-sensitivity C-reactive protein and Matrix metallo preteinases-9 were significantly decreased, high density lipoprotein cholesterol was increase. In contrast to control animals, in the pioglitazone-treated group, the change of the absolute plaque content of necrotic core decreased[ -0. 10 （0. 00,0. 10） vs. 0. 10（0. 00,0. 13 ）mm2 ,P 〈0. 001 ], as well as the relative plaque content of necrotic core [ - 2. 35 ± 3.11 ） vs. （ 1.87 ± 4. 62） %, P = 0. 012 ], absolute dense-calcium [ 0. 00 （0. 00,0. 10 ） vs. 0. 10（0. 00,0. l 0 ）mm2, P 〈0. 001 ], relative dense-calcium [ 0. 00 （ - 3.12,0. 14） vs. 2.00 （ -0. 19,3.19 ） %, P =0. 002 ]. The incidence of thrombotic events had significantly decreased in the pioglitazone group compared with the control group（ P 〈0. 001 ）. Conclution ： Pioglitazone, as an novel anti-atherosclerotic agent, may induce regression of atherosclerotic plaque and reduce the rate of atherothrombosis.
出处
《心肺血管病杂志》
CAS
2015年第6期491-495,共5页
Journal of Cardiovascular and Pulmonary Diseases
基金
北京市自然科学基金(项目编号:7132078)
关键词
吡格列酮
虚拟组织学血管内超声
动脉粥样硬化斑块
兔
Pioglitazone
Virtual histology intravascular ultrasound
Atherosclerotic plaque
Rabbit
