摘要
以豆腐黄水为液体培养基,酵母红-2为发酵菌种,研究不同培养条件对发酵生产酵母单细胞蛋白的影响,通过正交实验确定最佳发酵条件.实验结果表明:当豆腐黄水体积分数为70%、培养温度为30℃、接种量7%、装液量为50 m L/250 m L三角烧瓶、150 r/min摇床发酵培养37 h后,酵母单细胞蛋白产量可达4.15 g/L,其蛋白质量分数达27%以上.
With curb-farm waste water as the liquid medium and yeast red-two as the fermentation strain,the influence of different culture conditions on yeast fermentation to produce single cell protein is studied. By orthogonal experiments it is determined that the best condition for fermentation is that with curb-farm water concentration of 70 %,temperature of 30 ℃,inoculation amount of 7 %,liquid volume of 50 m L /250 m L,single-cell protein production can reach 4. 15 g / L,and protein content 27 %.
出处
《沈阳化工大学学报》
CAS
2015年第2期113-117,共5页
Journal of Shenyang University of Chemical Technology
基金
国家及辽宁省大学生创新创业训练计划项目(201310149014)
关键词
豆腐黄水
酵母菌
发酵
单细胞蛋白
bean curb-farm waste water
yeast
fermentation
single-cell protein
