摘要
目的研究瘢痕内注射脂肪来源干细胞(ADSC)对兔耳增生性瘢痕的抑制作用及可能机制。方法选取12只新西兰大白兔,制作兔耳增生性瘢痕模型,随机平均分成3组,2周后各组右耳瘢痕内注射DMEM作为自身对照,左耳分别注射ADSCs、脂肪来源干细胞条件培养基(ADSCs-CM)及不作处理。注射前及注射后1、2、3周检测瘢痕增生情况,并于注射后3周取材,行组织化学及基因学检测。结果造模2周后,伤口均完全上皮化;DMEM注射及未处理组的伤口逐渐出现增厚、变红、变硬等增生表现,注射后3周时最为明显;ADSCs及ADSCs-CM注射组均未出现明显增生反应。取材后HE、Masson染色示,ADSCs及ADSCs-CM注射组瘢痕的胶原密度适中、排列整齐;对照及未处理组瘢痕可见大量致密、杂乱的胶原组织。基因检测发现,ADSCs及ADSCs-CM注射组瘢痕的α-SMA、CollagenⅠ表达显著低于对照及未处理组。ADSCs注射组瘢痕冰冻切片Dil荧光染色可见大量存活脂肪来源干细胞。结论瘢痕内注射脂肪来源干细胞可以降低α-SMA、CollagenⅠ基因表达,从而改善瘢痕内胶原堆积,并最终改善瘢痕增生情况。
Objective To investigate the inhibition effect and mechanism of intra-cicatrix injection of adipose derived stem cells on hypertrophic scar in rabbit ear. Methods Twelve New Zealand white rabbits were used for establishing scar model and were randomly divided into 3 groups. Two weeks after the operation, all the right ears were injected DMEM as internal control while left ears of group 1, 2 were injected ADSCs, ADSCs-CM respectively. Left ears in the third group were remained untouched. Photos and ultrasonography were taken before and 1, 2, 3 weeks after injection. Histochemical and genetic detection were used 3 weeks after the rabbits were sacrificed for offering scar tissue. Results All wounds were re- epithelized 2 weeks after the injection. Wounds injected DMEM and untouched gradually grew thick, red and stiff which are the symptoms of hypertrophic scars, while ADSCs and ADSCs-CM injected ones showed no sign of growing hypertrophic. HE and Masson＇s staining showed collagen deposit and irregularly arrangement in the DMEM injection and untouched scars, while much less and better arranged collagen deposit were shown in both ADSCs and ADSCs-CM treated ones. Genetic detection showed lower expression of α-SMA, Collagen I in ADSCs and ADSCs-CM injection scars, compared with DMEM treated and untreated ones. Dil label staining showed a larger amount of ADSCs in the scar tissue of ADSCs treated group. Conclusion Intra-cicatrix injection of ADSCs can inhibit hypertrophic scar through ameliorating collagen deposit by down regulate the expression of α-SMA, Collagen I.
出处
《组织工程与重建外科杂志》
2015年第3期139-143,共5页
Journal of Tissue Engineering and Reconstructive Surgery
关键词
脂肪来源干细胞
增生性瘢痕
瘢痕内注射
Adipose derived stem cells
Hypertrophic scar
Intra-cicatrix injection
