摘要
从患病金鱼肝脏中分离获得一株细菌,编号为ZP-1,通过细菌形态学观察、理化特征、16SrDNA克隆测序以及系统进化树分析等方法进行鉴定。试验结果表明,ZP-1菌株为革兰氏阴性短杆菌,可以发酵葡萄糖、乳糖产酸、还原硝酸盐、产生吲哚等。进一步采用PCR方法扩增ZP-1菌株16SrDNA,将其亚克隆至pMD18-T载体中测序,获得长度为1464bp(登录号:KM458852),与鱼源致病性类志贺邻单胞菌(KC825322)同源性为99.80%,最终确定ZP-1菌株为类志贺邻单胞菌。采用30种抗生素进行药敏试验,ZP-1菌株对头孢噻肟、头孢克肟、头孢孟多、庆大霉素、丁胺卡那霉素、萘啶酸、氯霉素、氧氟沙星等药物敏感。
A bacterium was isolated from hepatopancreas in goldfish（Carassius auratus）and named ZP 1. The isolate was identified by bacterial morphology, physical and chemical characteristics, 16S rDNA se quencing and phylogenetic analysis. The results showed that ZP-1 strain is Gram-negative short-rod shaped bacillus, which was positive for acid production from glucose and lactose, reduction of nitrate, and indole production. The 16S rDNA of ZP-1 strain was amplified by PCR, and subcloned into pMD18-T vector, whose sequencing was 1464 bp in length （accession number. KM458852）. Phylogenetic tree showed that strain ZP-1 had high homology of 99.80O/oo with fish pathogenic Shiga Plesiomonas （KC825322） indicating that the isolate ZP-1 was identified as P. shigelloides. The susceptibility testing was performed using 30 kinds of antibiotics, and results showed that strain ZP 1 was sensitive to cefotaxime, cefixime, cefaman dole, gentamicin, amikacin, nalidixic acid, chloramphenicol and ofloxacin.
出处
《水产科学》
CAS
CSCD
北大核心
2015年第6期375-379,共5页
Fisheries Science
基金
国家自然科学基金资助项目(31272692)
江苏省青蓝工程项目(2014)
徐州科技局资助项目(XF12C027)
江苏省研究生创新工程项目(KYLX_1430)
关键词
金鱼
类志贺邻单胞菌
16SrDNA
药敏试验
goldfish
Plesiomonas shigelloides
isolation and identification
16S rDNA
antibiotic sensitivity
