摘要
为探寻桑椹菌核病化学防控药剂并分析其桑椹中农药的残留量,以桑椹肥大性菌核病病原菌为材料,用市售农药对该菌的初始传染源——子囊孢子进行了抑菌实验,实验结果显示:甲基硫菌灵和腐霉利对子囊孢子抑制效果最好,其EC50分别为0.015 5mg/mL和0.015 3mg/mL.经大田防治实验,结果表明,在适时交替喷洒甲基硫菌灵和腐霉利,对桑椹菌核病的防治效果高达97.96%±0.2%,而对照区发病率高达40.93%±0.2%.对化学防控实验区成熟桑椹,分别采用HPLC和GC对其农药残留进行分析,甲基硫菌灵的残留量超过国际标准限量,而腐霉利的残留量则在国际标准限量之内.其研究结果对改进桑椹菌核病的防控具有重要参考意义.
In order to provide useful information for the improvement of mulberry fruit sclerotiniosis pre-vention and control with chemical agents in mulberry gardens ,a few pesticides were tested and the resi-dues in mulberry fruit were determined .In an inhibition test ,several commercially available pesticides were used to treat the ascospores of hypertrophy sorosis sclerotenisis - the initial source of infection of the disease ,and the results showed that thiophanate-methyl and procymidone had the best inhibitory effect on their grow th ,their EC50 value being 0.015 5 mg /mL and 0.015 3 mg/mL ,respectively .In a field control experiment ,timely alternate spay of thiophanate-methy and procymidone gave a control effect on mulberry fruit sclerotiniosis of up to 97.96% ,while a high incidence of 40.93% was recorded in the control plot . HPLC and GC were used for the analysis of the pesticide residues in the ripe mulberry fruit .Thiophanate-methyl residues exceeded the national standard limit ,and procymidone residues were within the limits of national standards .
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第10期49-54,共6页
Journal of Southwest University(Natural Science Edition)
基金
中央高校基本业务专项资金项目(XDJK2014D016)
南部县蚕桑局校地合作项目
国家现代农业产业技术体系建设专项(CARS-22)
关键词
桑椹菌核病
化学防控
农药残留
高效液相色谱
气相色谱
mulberry fruit sclerotiniosis
chemical prevention and control
pesticide residue
high perform-ance liquid chromatography
gas chromatography
