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脊髓背角P2X_4、P2Y_(12)和P2Y_(13)受体参与AM1241对CCI大鼠的镇痛效应 被引量：2

P2X_4,P2Y_(12),P2Y_(13) receptors in spinal dorsal horn are involved in AM1241-induced analgesia in rats with chronic constriction injury
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摘要 目的观察鞘内注射大麻素CB2受体激动剂AM1241对慢性坐骨神经结扎(CCI)大鼠机械痛阈、脊髓背角P2X4、P2Y12和P2Y13受体表达变化的影响。方法健康成年雄性Sprague-Dawley大鼠40只随机分为5组(n=8):假手术组、CCI组(坐骨神经结扎+鞘内注射0.005%DMSO生理盐水)、1 pmol/LAM1241处理组(坐骨神经结扎+鞘内注射AM1241 1pmol/L)、10 pmol/LAM1241处理组(坐骨神经结扎+鞘内注射AM1241 10 pmol/L)、100 pmol/LAM1241处理组(坐骨神经结扎+鞘内注射AM1241 100 pmol/L)。CCI组及不同浓度AM1241处理组大鼠均于鞘内置管7 d之后行坐骨神经结扎,连续14 d鞘内注射,每天2次;分别在术前1 d,术后第1、3、5、7、10、14天注射1 h后测定机械痛阈(MWT),分别在术后第7,14天免疫印迹法(western blot)观察脊髓背角P2X4、P2Y12和P2Y13受体表达变化。结果 1CCI组术后第1、3、5、7、10、14天MWT显著低于假手术组(P<0.05),10 pmol/L AM1241和100 pmol/L AM1241处理组术后第1、3、5、7天MWT明显高于CCI组(P<0.01);但10 pmol/L AM1241处理组术后第10、14天MWT与CCI组相比无明显差异(P>0.05);100 pmol/L AM1241处理组术后第10、14天MWT仍高于CCI组(P<0.05);2与假手术组相比,CCI组术后第7、14天脊髓背角P2X4、P2Y12和P2Y13受体表达显著上调(P<0.01);与CCI组相比,100 mol/L AM1241处理组在术后第7天抑制脊髓背角P2Y12和P2Y13受体表达(P<0.05),在术后第14天抑制背角P2X4受体表达(P<0.05)。结论大麻素CB2受体激动剂AM1241的镇痛效应可能与抑制脊髓背角小胶质细胞P2X4、P2Y12和P2Y13受体表达有关。 Objective To investigate the effect of intrathecal injection AM1241,a selective CB2（Cannabinoid receptor 2） receptor agonist,on mechanical withdrawl threshold（MWT） and the expression of P2X4,P2Y12 and P2Y13receptors in spinal dorsal horn of rats with chronic constriction injury（CCI） of the sciatic nerve.Methods Forty male Sprague-Dawley rats were randomly divided into five groups（n = 8） ：sham group（sham operation）,CCI group（CCI ＋ intrathecal injection of 0.005% DMSO saline solution）,1 pmol / L AM1241 group,（CCI ＋ intrathecal injection of 1 pmol/L AM1241）,10 pmol/L AM1241 group（CCI ＋ intrathecal injection of 10 pmol / L AM1241）,100 pmol / L AM1241 group（CCI ＋ intrathecal injection of 100 pmol / L AM1241）.Rats in CCI group and all AM1241 groups were treated with with chronic constriction injury of the sciatic nerve after 7d of intrathecal tude.The intrathecal injection was administered twice a day for 14 days.MWT were evaluated at1,3,5,7,10 and 14 d after surgery.The expression of P2X4,P2Y12 and P2Y13receptors in dorsal horn was assessed by western blot at 7 and 14 post-operative days.Results 1） MWT in CCI group was significantly lower than sham group at 1,3,5,7,10 and 14 d after surgery（P 0.05）.MWT in 10 pmol / L AM1241 group was markedly longer than CCI group at 1,3,5,7 d（P〈 0.01）.MWT in 100 pmol / L AM1241 group was significantly longer than CCI group at 1,3,5,7 d and 14 d after surgery（P 〈0.01 at 1,3,5,7 post-operative days;P 〈0.05 at 10,14 post-operative days）.2） Compared with the sham group,the P2X4,P2Y12 and P2Y13receptors expression in CCI group were markedly enhanced on 7d and 14 d after surgery（P 〈0.01）.Compare with the CCI group,the expression of P2Y12 and P2Y13in 100 pmol / L AM1241 group was suppressed on 7d（P 〈0.01）,the expression of P2X4 in 100 pmol / L AM1241 group was suppressed on 14 d after surgery（P〈 0.01）.Conclusion Analgesia effect of cannabinoid CB2 receptor agonist AM1241 may be related to the inhibition of the expression of P2X4,P2Y12 and P2Y13receptors in the spinal dorsal horn microglial cells.
作者 田虹 黄婷婷 刘正律 陈远寿 刘晓红 曾俊伟
机构地区 遵义医学院基础医学院生理学教研室 贵州省麻醉与器官功能保护重点实验室
出处 《遵义医学院学报》 2015年第3期230-234,238,共6页 Journal of Zunyi Medical University
基金 2012教育部科学技术重点项目(NO:2012125) 2010年贵州省科技厅资助项目(NO:黔科合J字(2010)2264) 遵义医学院重点学科项目(NO:XZXK-20120702) 遵义医学院大学生创新训练重点项目(NO:院发[2014]5807)
关键词 神经病理性疼痛 CB2受体 小胶质细胞 neuropathic pain CB2 receptor microglial
分类号 R347.7 [医药卫生—基础医学]
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参考文献18

  • 1Racz I, Nadal X, Alferink J,et al. Crucial role of CB2 cannabinoid receptor in the regulation of central immune responses during neuropathic pain[J]. J Neurosci,2008,28(46):12125-12135.
  • 2Wilkerson J L, Gentry K R, Dengler E C, et al. Immunofluorescent spectral analysis reveals the intrathecal cannabinoid agonist, AM1241, produces spinal anti-inflammatory cytokine responses in neuropathic rats exhibiting relief from allodynia[J]. Brain Behav,2012, 2(2): 155-177.
  • 3Kobayashi K, Yamanaka H, Yanamoto F, et al. Multiple P2Y subtypes in spinal microglia are involved in neuropathic pain after peripheral nerve injury[J]. Glia, 2012, 60(10): 1529-1539.
  • 4李敏,肖菊平,曾俊伟,肖智,陈远寿,田虹,刘晓红.脊髓糖皮质激素受体在神经病理性疼痛中的作用[J].第三军医大学学报,2012,34(17):1719-1721. 被引量：7
  • 5McGaraughty S, Wismer C T, Zhu C Z,et al. Effects of A-317491, a novel and selective P2X3/P2X2/3 receptor antagonist, on neuropathic, inflammatory and chemogenic nociception following intrathecal and intraplantar administration[J]. Br J Pharmacol, 2003,140(8):1381-1388.
  • 6Naguib M, Xu J J, Diaz P, et al. Prevention of paclitaxel-induced neuropathy through activation of the central cannabinoid type 2 receptor system[J]. Anesth Analg, 2012, 114(5): 1104-2011.
  • 7Wilkerson J L, Gentry K R, Dengler E C, et al. Immunofluorescent spectral analysis reveals the intrathecal cannabinoid agonist, AM1241, produces spinal anti-inflammatory cytokine responses in neuropathic rats exhibiting relief from allodynia[J].Brain Behav,2012, 2(2):155-177.
  • 8Deng L, Guindon J, Cornett B L,et al.Chronic cannabinoid receptor 2 activation reverses paclitaxel neuropathy without tolerance or cannabinoid receptor 1-dependent withdrawal[J].Biol Psychiatry,2015,77(5):475-487.
  • 9Sun Y, Zhang W, Liu Y,et al. Intrathecal injection of JWH015 attenuates remifentanil-induced postoperative hyperalgesia by inhibiting activation of spinal glia in a rat model[J].Anesth Analg,2014,118(4):841-853.
  • 10Dieb W, Hafidi A. Astrocytes are involved in trigeminal dynamic mechanical allodynia: potential role of D-serine[J]. J Dent Res, 2013, 92(9): 808-813.

二级参考文献10

  • 1Liu X H, Zeng J W, Zhao Y D, et al. Rapid inhibition of ATP- induced currents by corticosterone in rat dorsal root ganglion neurons [J]. Pharmacology, 2008, 82(2): 164- 170.
  • 2Mao J. Central glucocorticoid receptor: a new role in the cellular mecha- nisms of neuropathic pain[J]. Rev Neurosci, 2005, 16(3) : 233 -238.
  • 3Alexander J K, DeVries A C, Kigerl K A, et al. Stress exacerbates neuropathic pain via glucocorticoid and NMDA receptor activation[ J]. Brain Behav Immun, 2009, 23(6) : 851 -860.
  • 4Wang S, Lim G, Zeng Q, et al. Central glucocorticoid receptors modu- late the expression and function of spinal NMDA receptors after periph- eral nerve injury[J]. J Neurosci, 2005, 25(2): 488 -495.
  • 5Bennett G J, Xie Y K. A peripheral mononeuropathy in rat that pro- duces disorders of pain sensation like those seen in man[J]. Pain, 1988, 33(1) : 87 -107.
  • 6Hargreaves K, Dubner R, Brown F, et al. A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia[ J]. Pain, 1988, 32(1) : 77 -88.
  • 7Ulrich-Lai Y M, Xie W, Meij J T, et al. Limbic and HPA axis func- tion in an animal model of chronic neuropathic pain [ J ]. Physiol Be- hay, 2006, 88(1/2): 67-76.
  • 8Myers B, Greenwood-Van-Meerveld B. Divergent effects of amygdala glucoeortieoid and mineralocorticoid receptors in the regulation of vis- ceral and somatic pain[J]. Am J Physiol Gastrointest Liver Physiol, 2010, 298(2) : G295 - G303.
  • 9Capasso A, Loizzo A. Functional interference of dexamethasone on some morphine effects: hypothesis for the stcroid-opioid interaction [J]. Recent Pat CNS Drug Discov, 2008, 3(2) : 138 -150.
  • 10刘晓红,曾俊伟,肖智,阮怀珍.地塞米松快速抑制大鼠背根神经节细胞中ATP所致的钙升高[J].第三军医大学学报,2009,31(14):1329-1332. 被引量：5

共引文献6

同被引文献21

  • 1Lih C C,Sherry S J H,Yu C H. Targeting the cannabinoidsystem for pain relief? [ J]. Acta Anaesthesiologica Tai-wanica,2013 ,51(4) : 161 -170.
  • 2Enza P, Livio L, Vito de N. The role of cannabinoid re-ceptors in the descending modulation of pain[ J]. Pharma-ceuticals ,2010,3(8) : 2661 -2673.
  • 3Lu C,Liu Y,Sun B, et al. Intrathecal injection of JWH-015 attenuates bone cancer pain via time - dependentmodification of pro - inflammatory cytokines expressionand astrocytes activity in spinal cord [ J]. Inflammation,2015,38(5) : 1880-1890.
  • 4Xu J J, Diaz P,Bie B, et al. Spinal gene expression pro-filing and pathways analysis of a CB2 agonist ( MDA7 )-targeted prevention of paclitaxel - induced neuropathy[J]. Neuroscience, 2014,28(260) : 185 -194.
  • 5Landry R P,Martinez E, de Leo J A, et al. Spinal can-nabinoid receptor type 2 agonist reduces mechanical alio-dynia and induces mitogen - activated protein kinase phos-phatases in a rat model of neuropathic pain [ J ]. J Pain,2012,13(9) : 836 -848.
  • 6Old E A,Clark A K, Malcangio M. The role of glia inthe spinal cord in neuropathic and inflammatory pain [ J ].Handb Exp Pharmacol,2015,227: 145 - 170.
  • 7Tsuda M. Mechanisms underlying the pathogenesis ofneuropathic pain revealing by the role of glial cells [ J ].Nihon Shinkei Seishin Yakurigaku Zasshi, 2015 ,35( 1 );1 -4.
  • 8Tumati S,Largent - Milnes T M,Keresztes A, et al. Re-peated morphine treatment - mediated hyperalgesia,allo-dynia and spinal glial activation are blocked by co - ad-ministration of a selective cannabinoid receptor type - 2agonist [ J ]. J Neuroimmunol,2012,244 ( 1 - 2 ) : 23 -31.
  • 9Davis M P. Cannabinoids in pain management : CBI,CB2 and non - classic receptor ligands[ J]. Expert OpinInvestig Drugs,2014,23(8) : 1123 -1140.
  • 10Farquhar - Smith W P,Egertovd M, Bradbury E J, etal. Cannabinoid CB( 1 ) receptor expression in rat spinalcord[J]. Mol Cell Neurosci,2000,15(6) : 510-521.

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遵义医学院学报
2015年 第3期

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