摘要
目的研究维药神香草水蒸气蒸馏提取挥发油与超临界CO2流体萃取物化学成分的异同。方法采用水蒸气蒸馏法与超临界CO2流体萃取法分别提取神香草挥发油和超临界CO2流体萃取物,并通过气相色谱-质谱(GC-MS)联用仪对其化学成分进行分析和鉴定。结果维药神香草挥发油的水蒸气蒸馏法提取率为0.68%,GC-MS分析、鉴定出所有65种化合物,其中含量较高的是亚油酸(13.83%)、棕榈酸(13.80%)、(+)-胡薄荷酮(8.31%)、(+)斯巴醇(6.27%)、二十一烷(5.34%)、香茅酸(4.07%)、二十六烷(4.02%)、二十四烷(3.28%)、二十烷(3.06%)。维药神香草超临界CO2流体萃取物的萃取得率为5.5%,GC-MS分析、鉴定出所有34种化合物,其中γ-谷甾醇(31.882%)、二十八烷(19.953%)、亚麻酸(16.279%)、三十六烷(15.939%)、棕榈酸(10.658%)、亚麻酸乙酯(12.471%)、三十一烷(12.215%)、9,12,15-十八烷三烯酸乙酯(9.057%)化合物含量较高。结论维药神香草挥发油与超临界CO2流体萃取物化学组成存在较大的差异,超临界CO2流体萃取物中的γ-谷甾醇、亚麻酸、亚麻酸乙酯等被认为是具有较强生物活性的化合物,且含量较高,具有较大的潜在研究前景。
Objective To study the similarity and differences in chemical composition and relative content of volatile oil by steam distillation and supercritical CO2 Fluid extraction extracts of Hyssopus officinalis L. （Hyssop）,a Traditional Uighur Hreb Medicine.Methods Hyssopus volatile oil and SFE exctracts was extracted by steam distillation and supercritical CO2 Fluid extraction methods and the gas chromatography-mass spectrometry （GC-MS）were used to analyze its chemical composition.Then,relative content of each component has been calculated with a peak area normalization method.Results There were 65 components separated and identified from volatile oil of Hyssop by team distillation,and its content was 0.68%,which mainly included Linoleic acid （13.83%）,palmitic acid （13.80%）,（＋）-pulegone（8.31%）,（＋）Spartak alcohol （6.27%）,heneicosanoic （5.34%）,lemongrass acid （4.07%）,hexacosanoic （4.02%）,lignoceric （3.28%）,eicosanoic （3.06%）.There were 34 components separated and identified from SFE exctracts of Hyssop by supercritical CO2 Fluid extraction,and the content reached 5.5% ,which mainly includedγ-si-tosterol （31.882%）,heneicosanoic （19.953%）,linolenic acid （16.279%）,hexatriacontanoic （15.939%）, palmitic acid （10.658%）,flax ethyl （12.471%）,hentriaconta （12.215%）,9,12,15 octadecane leukotriene ethyl （9.057%）.Conclusion The result showed that there were many differences in chemical composition and relative content of Hyssop extractions from steam distillation and supercritical CO 2 Fluid extraction. We find some higher content of chemical compounds such as γ-sitosterol,linolenic acid and flax ethyl in the supercritical CO2 Fluid extraction which are considered biologically active compounds and have great potential research prospects.
出处
《新疆医科大学学报》
CAS
2015年第7期823-827,共5页
Journal of Xinjiang Medical University
基金
新疆少数民族科技人才特殊培养计划科研项目(201323122)
