摘要
目的探讨祛瘀化痰方对动脉粥样硬化易损斑块模型大鼠的抗炎作用。方法将SD大鼠随机分为假手术组、模型对照组、阿托伐他汀钙组、祛瘀化痰方低剂量组和祛瘀化痰方高剂量组5组。假手术组给予普通饲料,其余各组采用维生素D3联合高脂饲料喂养,每天按相应剂量灌胃给药。给药两周后测定血脂,达到高血脂水平后进行大鼠右侧颈动脉内皮拉伤手术致易损斑块,给药6周后,麻醉大鼠取血制备血清和血浆,用酶联免疫吸附法检测血浆中同型半胱氨酸(Hcy)、血清中细胞间黏附分子-1(ICAM-1)和血管细胞黏附分子-1(VCAM-1)水平。取大鼠右侧颈总动脉拉伤中段切片,采用苏木素-伊红(HE)染色,光镜观察大鼠右侧颈总动脉病理组织学改变。结果祛瘀化痰方高、低剂量组Hcy、ICAM-1、VCAM-1水平均比模型对照组明显降低(P<0.01)。HE染色显示模型对照组有明显的斑块形成,并有大量炎性细胞及泡沫细胞浸润,管腔狭窄,并伴有钙化及坏死区域。祛瘀化痰方高、低剂量组与模型组比较斑块面积减少,坏死程度减轻,且高剂量组效果优于低剂量组。结论祛瘀化痰方对易损斑块模型大鼠的炎症有明显抑制作用,可能是稳定易损斑块模型大鼠作用机理之一。
Objective： To observe the influence of Quyu Huatan Decoction on the inflammation of vulnerable plaque rats. Methods： Rats were randomly divided into 5 groups including the sham-operated group,the model group,the Atorvastatin group,the herbal medicine low-dose group and the herbal high-dose group. Except Shamoperated group,other groups were intragastric administration with vitamin D3 and fed with high fat diet. The vulnerable atherosclerotic plaque model was reproduced by operation on carotid artery endothelium for inducing MCAO nylon suture pull injury. At the end,the blood sample was collected to measure the concentration of Hcy,ICAM-1,VCAM-1. And arteries were collected for paraffin sections. Paraffin sections were stained by hematoxylin-eosin（HE）. Results： Compared with model group,the contents of Hcy,ICAM-1,VCAM-1 were reduced in herbal medicine low-dose group and high-dose group（P〈0.01）. HE staining results showed that the right common carotid artery intimal of sham-operated group was smooth and complete. Compared with the sham-operated group,there were some atherosclerotic plaques in model group. A large number of inflammatory cells and foam cells were visible in intima. There were some necrosis and calcification areas in severe places. Compared with the model group,the areas of atherosclerotic plaques and the stenosis extent of carotid artery were reduced in herbal medicine low and high dose group. And the effect of high dose group was better than those of the low dose group. Conclusion： Quyu Huatan Decoction has inhibitory effect on inflammation on vulnerable plaque rats.
出处
《中国中医急症》
2015年第5期784-786,790,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
首都中医药研究专项(14ZY11)
