摘要
目的:通过检测脊髓损伤后运动功能恢复情况和巢蛋白(nestin)、神经生长因子(NGF)的蛋白表达来探讨电针(EA)结合减重步行训练疗法(BWSTT)干预脊髓损伤(SCI)的作用。方法:选用健康成年清洁级雄性SD大鼠72只,随机分为假手术对照组(假手术组)、模型对照组(模型组)、电针治疗组(电针组)、电针结合减重步行训练治疗组(电针+训练组)。用美国NYU脊椎冲击损伤仪致大鼠T9—T10段脊髓急性中度损伤模型。BBB运动功能评分对大鼠后肢运动功能的恢复情况进行评估;免疫组织化学技术检测各时间点损伤段脊髓NGF和nestin的表达。结果:与模型组相比,两治疗组BBB评分显著增加,电针结合减重步行训练组在术后第14天和第28天显著增加,与电针组比较具有显著性差异(P<0.05)。两治疗组脊髓损伤术后第14天和第28天NGF和Nestin的表达显著增加,但二者没有显著性差异(P>0.05)。结论:1电针能够促进脊髓损伤大鼠内源性NGF和nestin的大量表达来促进神经再生。2电针结合减重步行训练对大鼠运动功能的恢复更为有效。
Objective： To investigate the locomotor functional recovery and its mechanism of electroacupuncture（EA） combined with body weight support treadmill training（BWSTT） treatment on spinal cord injury（SCI） in rats by testing the expressions of nestin and nerve growth factor（NGF） in the spinal lesion segment.Method： Sprague- Dawley rats were randomly divided into the sham operated control group（Shamcontrol group）, the operated control group（Op- control group）, electroacupuncture treatment group（EA group） and EA combined with BWSTT treatment group（EA with BWSTT group）. Spinal cord contusion model was made at T10 segments with NYU impactor. Behavioral test was evaluated by Basso- Beattie- Bresnahan（BBB） scale of hind limb movement. Furthermore, the expressions of nestin and NGF were determined by immunohistochemical method.Result： BBB scores increased significantly in the two treatment groups compared with the Op-control group and the BBB scores of EA with BWSTT group was significant higher compared with the EA group in the14th and 28 th d post- operation. In EA and EA with BWSTT group, the expressions of NGF and nestin increased significantly in two treatment groups at the 7th d, 14 th d and 28 th d after operation. however, there was no statistic difference between two treatment groups.Conclusion： EA can increase the expressions of endogenous NGF and nestin post-SCI so as to promote the nerve regeneration. EA combined with BWSTT treatment is more effective in locomotor functional recovery.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2015年第6期532-537,共6页
Chinese Journal of Rehabilitation Medicine
基金
国家自然科学基金资助项目(81102644)
浙江省自然科学基金资助项目(Y2100533)
国家中医药管理局中医药重点学科(针灸学)建设经费资助(国中医药发[2009]30)
浙江省大学生科技创新项目(2013R410010)
关键词
脊髓损伤
电针
减重步行训练
巢蛋白
神经生长因子
pinal cord injury
electroacupuncture
body weight support treadmill training
nestin
nerve growth factor
